Sc 7951

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-7951 is an antibody product offered by Santa Cruz Biotechnology. It is a research-use only tool for immunological applications. The core function of Sc-7951 is to serve as a detection reagent in various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Complete protease inhibitor tablet, by Merck Group (1 mentions) Ab134175, by Abcam (1 mentions) Histostain plus 3rd gen ihc detection kit, by Thermo Fisher Scientific (1 mentions) Deadend colorimetric tunel system, by Promega (1 mentions) Ab52298, by Abcam (1 mentions) Ab133575, by Abcam (1 mentions) Cs 9662, by Cell Signaling Technology (1 mentions) Cs 4970, by Cell Signaling Technology (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Radioimmunoprecipitation assay buffer, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride nylon membranes, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Ab10148, by Abcam (1 mentions) Sc 376861, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

COX-2 (sc-7951), (2 citations)

3 protocols using sc 7951

Comprehensive Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Naproxen (>99% purity; TCI America, Oregon, USA), EDTA, EGTA, Sodium orthovanadate, Igepal CA-630, Triton X-100,) Complete Protease inhibitor tablet and PhosSTOP phosphate inhibit tablet (Sigma) were purchased. Primary antibodies against PCNA (ab-29), cyclin D1 (ab-134175), caspase-9 (ab-52298), and IL-10 (ab-133575) were purchased from Abcam (MA, USA). Antibodies against caspase-3 (cs-9662), p21 (cs-2947), PARP (cs-9542), actin (cs-4970), cre (cs-15036s), RalA (cs-4799s), PI3K (cs-4292s), and horseradish-peroxidase-conjugated secondary antibodies (anti-rabbit, -goat, and –mouse) were purchased from Cell Signaling Technology (Beverly, MA, USA). Primary antibody against p21 (sc-397), caspase-3 (sc-397; for immunohistochemistry [IHC]), and COX-2 (sc-7951) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Prostaglandin E₂ ELISA kit (Cat. No. 514010) and mouse CXC-Chemokine receptor 4 (CXCR4) ELISA kit (Cat. No. MBS701736) were purchased from Cayman Chemical Company (Ann Arbor, MI) and MyBiosource.com (San Diego, USA). The Histostain-Plus 3^rd Gen IHC Detection Kit (Life Technologies, NY, USA) and DeadEnd Colorimetric TUNEL system (Promega, Wisconsin, USA) were purchased.

Kumar G., Madka V., Singh A., Farooqui M., Stratton N., Lightfoot S., Mohammed A, & Rao C.V. (2021). Naproxen inhibits spontaneous lung adenocarcinoma formation in KrasG12V mice. Neoplasia (New York, N.Y.), 23(6), 574-583.

+ Open protocol

+ Expand

Chondrocyte Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

HACs were lysed in radioimmunoprecipitation assay buffer (Invitrogen) supplemented with protease inhibitor cocktail (Sigma–Aldrich). After lysis, total proteins were separated in a NuPAGE Novex 4–12% Bis-Tris Gel (Invitrogen) and transferred to polyvinylidene difluoride nylon membranes (Sigma–Aldrich). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline and incubated overnight at 4 °C with primary antibodies against COX-2 (sc-7951; Santa Cruz Biotechnology, CA, USA), Col10a1 (bs0554 R; Bioss, MA, USA), PTHrP (TA334682; OriGene Technology, MD, USA) and GAPDH (MA5-15738; Thermo Scientific, MA, USA). Specific bands were detected with a horseradish peroxidase-conjugated secondary antibody and were read using an enhanced chemiluminescence western blot system from Pierce (Rockford, IL, USA).

Chang L.H., Chen C.H., Wu S.C., Chang J.K, & Ho M.L. (2021). Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats. Journal of Orthopaedic Translation, 30, 16-30.

+ Open protocol

+ Expand

Quantification of Amyloid-Beta Peptides in Mouse Brain and Retina

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western gels and enzyme-linked immunoabsorbent assay (ELISA) were performed for quantification of Aβ40 and Aβ42 peptides and total Aβ40 + Aβ42 peptide load in control and transgenic mouse brain and retina as previously described [18 (link)–20 (link),24 (link)–26 (link)]. Western immunoblots were performed using murine- and/or human-specific primary antibodies directed against Aβ40 or Aβ42 peptide (BDI350; ab20068 or ab10148; Abcam, Cambridge, MA, USA), the control protein marker β-actin (3598–100; Sigma-Aldrich Chemical Company, St. Louis, Missouri, USA), CRP (N-14; sc-18304), CRP (H-90; sc-30047) and/or CRP (C-13; sc-18306), COX-1 (AS70; sc-52757 and/or 41272; sc-70878), COX-2 (H3; sc-376861 and/or H-62; sc-7951; Santa Cruz Biotechnologies, CA, USA) using methods previously described by our group [18 (link)–27 ]. Both COX-2 and CRP levels were initially compared to an unchanging internal β-actin in the sample and then these values were used to derive COX-2 and CRP relative expression levels. Three different antibodies were used for CRP detection and COX-1 was used as the control for COX-2 as previously described [29 (link)].

Pogue A., Dua P., Hill J, & Lukiw W. (2015). Progressive inflammatory pathology in the retina of aluminum-fed 5xFAD transgenic mice. Journal of inorganic biochemistry, 152, 206-209.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!