Log In Sign up
The largest database of trusted experimental protocols

Snord 72

Manufactured by Qiagen
Visit Supplier

Snord 72 is a small nucleolar RNA (snoRNA) that is involved in the modification of other cellular RNAs. snoRNAs play a crucial role in the processing and modification of various types of RNAs, including ribosomal RNA, small nuclear RNA, and small Cajal body-specific RNA. The core function of Snord 72 is to guide the site-specific modification of target RNAs, which is an important step in their maturation and function.

Automatically generated - may contain errors

Lab products found in correlation

Snord68, by Qiagen (1 mentions) Cfx96 system, by Bio-Rad (1 mentions) Mir 146a, by Qiagen (1 mentions) Miscript sybr green pcr kit, by Qiagen (1 mentions) Miscript 2 rt kit, by Qiagen (1 mentions) Mirneasy kit, by Qiagen (1 mentions) Stratagene mx3000p, by Agilent Technologies (1 mentions)

2 protocols using snord 72

1

Quantifying miR-142 Expression in Cells and Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
MicroRNAs (miR-142-3p and miR-142-5p) and their predicted target levels were measured by real-time reverse transcription–PCR using SYBR Green dye on a Bio-Rad CFX96 system in cells and tissues. MicroRNA expression data were normalized against snord 68 and snord 72 expression levels (Qiagen). Expression of the other genes were normalized against β-actin mRNA levels. Primer sequences used for mRNA expression analysis are shown in Additional file 1: Table S1.
Talebi F., Ghorbani S., Chan W.F., Boghozian R., Masoumi F., Ghasemi S., Vojgani M., Power C, & Noorbakhsh F. (2017). MicroRNA-142 regulates inflammation and T cell differentiation in an animal model of multiple sclerosis. Journal of Neuroinflammation, 14, 55.
+ Open protocol
+ Expand
2

miRNA Expression Analysis in Brain Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from the brain tissue was isolated using miRNeasy Kit (Qiagen, Valencia, CA, USA) and converted to cDNA using miScript II RT kit (Qiagen), as per manufacturer’s instructions. The cDNA samples were amplified for determining different miRNAs expressions in a 96 well format RT2-qPCR array (MIMM-107ZA-2, Mouse Neurologic Development and Disease, Qiagen) using miScript SYBR Green PCR kit (Qiagen). The cDNA samples were also analyzed for individual miRNAs expression (miR-146a, snoRD72 and RNU-6) (Qiagen). The amplification was performed in Stratagene Mx3000p (Agilent Technologies, Santa Clara, CA) and the cycle threshold (CT) values were determined after baseline and threshold adjustments. For the analysis, baseline and threshold adjustments were kept constant for all the experiments and the results were expressed as fold expression. The altered miRNAs (up-regulated or down-regulated) were checked for sequence similarity to PrPc by an online software targetscan provided by Whitehead institute for the targets of miRNAs (http://www.targetscan.org/).
Kalani A., Chaturvedi P., Maldonado C., Bauer P., Joshua I.G., Tyagi S.C, & Tyagi N. (2017). Dementia-like pathology in type-2 diabetes: A novel microRNA mechanism. Molecular and cellular neurosciences, 80, 58-65.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!