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7 protocols using fluorolink md 700

1

Fabrication of Solid-State Lithium-Sulfur Electrolyte

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Example 1

In a glovebox operating under argon atmosphere, 2.4 g of lithium sulfide glass (Li2S:P2S5=75:25) was placed in a cup. 1.04 g of PFPE dimethacrylate (Fluorolink MD 700, Solvay) was added, followed by 0.051 g of benzoyl peroxide and 7.3 g of dry Fluorinert 70. The cup was placed in a Thinky mixer (Thinky ARV-SOLED) and mixed at 1500 rpm for 40 mins. The slurry was cast on aluminum foil using a doctor blade. The film was dried on the coater, while maintaining the vacuum, then was transferred to an antechamber and dried under vacuum at 60° C. The dried film was scraped off and the material was pressed into pellets under 120 MPa, using hydraulic press, and then heated at 125° C. for two hours. After that, the heating was removed, the pellet was cooled to room temperature and only then the pressure was released.

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2

Preparation of Lithium Sulfide Glass-Polymer Composite Electrolyte

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Example 1

In a glovebox operating under argon atmosphere, 2.4 g of lithium sulfide glass (Li2S:P2S5=75:25) was placed in a cup. 1.04 g of PFPE dimethacrylate (Fluorolink MD 700, Solvay) was added, followed by 0.051 g of benzoyl peroxide and 7.3 g of dry Fluorinert 70. The cup was placed in a Thinky mixer (Thinky ARV-50LED) and mixed at 1500 rpm for 40 mins. The slurry was cast on aluminum foil using a doctor blade. The film was dried on the coater, while maintaining the vacuum, then was transferred to an antechamber and dried under vacuum at 60° C. The dried film was scraped off and the material was pressed into pellets under 120 MPa, using hydraulic press, and then heated at 125° C. for two hours. After that, the heating was removed, the pellet was cooled to room temperature and only then the pressure was released.

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3

PFPE-Based Photosensitizer Delivery for Cancer

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PFPE (Fluorolink® MD700) was obtained from Solvay (Brussels, Belgium). Pluronic (Mn 2900) and dimethyl sulfoxide (DMSO) were provided by Aladdin Industrial Corporation (Shanghai, China). Chlorin e6 (Ce6) was purchased from Frontier Scientific (Logan, UT, USA). 1,3-diphenylisobenzofuran (DPBF) was purchased from TCI (Tokyo, Japan). PC-3 cells (prostate cancer cells) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Advanced Dulbecco’s modified Eagle’s medium (DMEM) medium was provided by Thermo Fisher Scientific (Bremen, Germany). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Double distilled water was used throughout the experiments.
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4

Preparation of Lithium Sulfide Glass-Polymer Composite Electrolyte

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Example 1

In a glovebox operating under argon atmosphere, 2.4 g of lithium sulfide glass (Li2S:P2S5=75:25) was placed in a cup. 1.04 g of PFPE dimethacrylate (Fluorolink MD 700, Solvay) was added, followed by 0.051 g of benzoyl peroxide and 7.3 g of dry Fluorinert 70. The cup was placed in a Thinky mixer (Thinky ARV-50LED) and mixed at 1500 rpm for 40 mins. The slurry was cast on aluminum foil using a doctor blade. The film was dried on the coater, while maintaining the vacuum, then was transferred to an antechamber and dried under vacuum at 60° C. The dried film was scraped off and the material was pressed into pellets under 120 MPa, using hydraulic press, and then heated at 125° C. for two hours. After that, the heating was removed, the pellet was cooled to room temperature and only then the pressure was released.

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5

Fabrication of PFPE Microfluidic Molds

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The UV-curable resin was prepared by mixing the PFPE resin (Fluorolink MD700, Solvay Solexis, Milan, Italy) with 3% w/w photoinitiator (Darocur 1173, Sigma-Aldrich, St. Louis, MO, USA). The UV-curable resin was dropped on the patterned silicon substrate (i.e., Si master) and covered with polyethylene terephthalate (PET) film as a backplane. To spread the UV-curable resin between the PET film and Si master, the PET film was rolled over the resin and cross-linked by UV irradiation at 365 nm for 5 min. After curing, the PFPE mold was detached from the Si master.
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6

Fabrication of Microfluidic Devices

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Chemicals and materials were obtained from the following sources and used without further purification: 2'-Deoxyadenosine-5'-monophosphate (Alfa Aesar); Thymidine-5'-monophosphate disodium salt (Alfa Aesar); 2'-Deoxycytidine-5'-monophosphate (Alfa Aesar); 2'-Deoxyguanosine-5'-monophosphate disodium salt hydrate (Alfa Aesar); potassium chloride (1 and 3 m, Sigma-Aldrich); Tris-EDTA (100×, Sigma-Aldrich); Tris-Borate-EDTA (10×, Sigma-Aldrich); silver (Ag) wires (>99%, Sigma-Aldrich); S1813 photoresist (MicroChem); MF319 developer (MicroChem); potassium hydroxide (KOH) pellets (Fisher Scientific); hydrofluoric acid (HF) (Sigma-Aldrich); Fluorolink MD 700 (Solvay); 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich); NOA72 (Norland Products); poly(ethylene glycol) diacrylate (PEGDA) (Mn = 250, Sigma-Aldrich); 2,2-dimethoxy-2-phenylacetophenone (Sigma-Aldrich); Si wafers (P/B, resistivity 5-10 Ωcm, orientation of (100), and 525 ± 25 μm thickness) (WaferPro); polyethylene terephthalate (PET) with 250 μm thickness (Goodfellow); polymethylmethacrylate (PMMA) with a thickness of 250 μm (Goodfellow); polymethylmethacrylate (PMMA) of 1 mm thickness (ePlastics).
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7

Microfluidic Device Fabrication Protocol

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PDMS elastomers and curing agents (Sylgard® 184) were purchased from Sewang Hitech (Gimpo, Republic of Korea). PFPE (Fluorolink MD 700, Solvay, Brussels, Belgium), PEG diacrylate (PEG-DA, Mn = 575, Sigma-Aldrich, St. Louis, MO, USA), and 2-hydroxy-2-methylpro-piophenone (Darocur 1173, Sigma-Aldrich) were used to fabricate the microfluidic device. Poly(vinyl alcohol) (PVA, Aldrich) was used as the surfactant for the continuous water phase. Isododecane (Alfa Aesar, Haverhill, MA, USA) was used for the oil phase.
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