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Hfls oa

Manufactured by Cell Applications
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The HFLS-OA is a laboratory equipment designed for performing high-frequency laser stimulation. It is a specialized device that generates and delivers high-frequency laser pulses for various experimental and research applications.

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Lab products found in correlation

Hfls ra, by Cell Applications (2 mentions) Penicillin streptomycin, by Cell Applications (1 mentions) Synoviocyte growth medium, by Cell Applications (1 mentions)

2 protocols using hfls oa

1

Culturing Rheumatoid Arthritis and Osteoarthritis Fibroblasts

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Three lots of human fibroblast-like synoviocytes from rheumatoid arthritis (HFLS-RA) patients and three from osteoarthritis (HFLS-OA) (Cell Applications, Inc.) patients were cultured separately in basal medium containing 10% growth supplement and 1% penicillin/streptomycin (Cell Applications, Inc.). Cells were incubated at 37 °C under 5% CO2. The medium was changed every 2 days. After confluence, the cells were seeded at a density of 3.0 × 105 cells/2 mL/well in α-MEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in 6-well plates.
Kisukeda T., Onaya J, & Yoshioka K. (2019). Effect of diclofenac etalhyaluronate (SI-613) on the production of high molecular weight sodium hyaluronate in human synoviocytes. BMC Musculoskeletal Disorders, 20, 201.
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2

Synoviocyte Activation in Osteoarthritis and RA

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Primary HFLS cells from healthy controls (HFLS control), osteoarthritis synovium (HFLS-OA), and RA synovium (HFLS-RA) were purchased from Cell Applications, Inc (San Diego, CA, USA). HFLS cells were grown to confluency in Synoviocyte Growth Medium (Cell Applications). Two independent sets of stimulations were performed on HFLS cells as outlined below.
Aripova N., Duryee M.J., England B.R., Hunter C.D., Mordeson J.E., Ryan E.M., Daubach E.C., Romberger D.J., Thiele G.M, & Mikuls T.R. (2023). Citrullinated and malondialdehyde-acetaldehyde modified fibrinogen activates macrophages and promotes an aggressive synovial fibroblast phenotype in patients with rheumatoid arthritis. Frontiers in Immunology, 14, 1203548.
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