Tryptone soya medium (agar, TSA; broth, TSB) and Lysogeny Broth (LB) medium were obtained from Oxoid Ltd. Tetracycline (Tc) and ciprofloxacin (CIP) antibiotics were supplied by Sigma-Aldrich; dimethyl sulfoxide (DMSO) and other analytical grade chemicals were supplied by Fisher Scientific. A minimal basal salts growth medium (BSM) was prepared as described by Rushton et al. [21 (link)] without casamino acids. A 3 : 1 cosmetics grade blend of chloromethylisothiazolinone and methylisothiazolinone (CMIT/MIT), and methylisothiazolinone (MIT) were obtained from Dow Chemical Company, USA; dimethyl dimethylol hydantoin (DMDMH) from Lonza, UK; benzisothiazolinone (BIT) and phenoxyethanol (PH) from Clariant International Ltd, Switzerland. Preservative stock solutions were prepared (in sterile distilled water or DMSO for PH) on the day of use and diluted as required.
Tryptone soya medium
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Tryptone soya medium is a general-purpose culture medium used for the growth and isolation of a wide range of microorganisms, including bacteria and fungi. It provides the necessary nutrients and growth factors for the cultivation of these microorganisms.
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Lab products found in correlation
2 protocols using tryptone soya medium
1
Antimicrobial Preservative Preparation Protocol
2
Triclosan Resistance in Aeromonas salmonicida
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The bacterial strains and plasmids used in this study are described in Table 1. E. coli strains DH5α and EPI-300 were routinely grown at 37°C in Luria-Bertani (LB) broth or LB agar, while A. salmonicida subsp. salmonicida strain 14791 (AS 14791) was grown at 25°C on tryptone soya medium (Oxoid, UK) supplemented with the desired antibiotics. The antibiotic concentration used for susceptibility testing was as follows: ampicillin 100 µg/ml; tetracycline 20 µg/ml; chloramphenicol 30 µg/ml; and Triclosan 1-100 µg/ml. Triclosan was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Fosmid pCC1FOS (Epicentre, USA) was used to construct the genomic library, whereas pUC119 and pRK415 were used for further subcloning experiments. The AS 14791 strain was obtained from the Korean Agricultural Culture Collection. In order to examine its resistance to Triclosan, this strain was grown on tryptone soya medium supplemented with 20 µg/ml of Triclosan.
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