For cell cycle kinetics analysis, after 7 days of treatment, cells were fixed in iced 70% ethanol and kept at −20 °C until reading on the flow cytometer. For reading, samples were resuspended in 200 μL of propidium iodide solution (5 µg/mL; Sigma-Aldrich), incubated for 30 min at room temperature, and analyzed in Guava CytoSoft 4.2.1 Software Environment (Guava Technologies) flow cytometer, counting at least 5000 events for each sample. The values were expressed as percentages of cells distributed at each phase of the cell cycle.
Guava cytosoft 4.2.1 software environment
Manufactured by Merck Group
Sourced in United States
The Guava CytoSoft 4.2.1 Software Environment is a data analysis software tool designed for use with Guava flow cytometry instruments. The software provides a platform for the acquisition, analysis, and management of flow cytometry data.
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Lab products found in correlation
2 protocols using guava cytosoft 4.2.1 software environment
1
Cell Cycle Kinetics Analysis
2
Annexin V-Based Cell Death Assay
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The analysis of cell death induction was performed with eBioscience™ Annexin V Apoptosis Detection Kits (Invitrogen, Waltham, MA, USA) evaluated by flow cytometry, according to the manufacturer’s protocol, using Guava EasyCyte MiniSystem device (Guava Technologies, Hayward, CA, USA) and analyzed by Guava CytoSoft 4.2.1 Software Environment (Guava Technologies). Cells were analyzed after 7 days of treatment, and the results were presented as percentages of Annexin-V positive cells. In addition, necrotic cells were also counted in the same assay.
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