Fitc anti mouse cd3 antibody 17a2

Mouse spleen cells were isolated from Parp9^+/+ (WT) and Parp9^-/- (KO) mice infected with VSV for 1 day. The cells were then fixed and stained with Zombie Aqua fixable viability kit (423102, Biolegend), APC/Cyanine7 anti-mouse CD45 antibody (30-F11, Biolegend), FITC anti-mouse CD3 antibody (17A2, Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (RM4-5, Biolegend), PerCP/Cyanine5.5 anti-mouse CD8a antibody (53-6.7, Biolegend) and APC anti-mouse CD19 antibody (1D3/CD19, Biolegend) (1 μl antibody for 2 million cells) for analyzing composition of CD4⁺, CD8⁺ and B cells. Flow cytometry data were acquired on a LSR-II flow cytometer (Beckton Dickinson) and analyzed using FlowJo v10 software (Tree Start)^{34 (link)}.

The primary antibodies for immonofluorescence were anti-Ki-67 rabbit monoclonal antibody (GB11030, dilution: 1:200) from Servicebio, anti-alpha smooth muscle actin antibody [E184] (ab32575, dilution: 1:200), anti-CD31 antibody [P2B1] (ab24590, dilution: 1:200), and anti-glucose transporter GLUT1 antibody [EPR3915] (ab115730, dilution: 1:200) from Abcam. Fibrinogen alpha chain polyclonal antibody (20645-1-AP, dilution: 1:100) was purchased from Proteintech. FITC anti-mouse CD3 antibody [17A2] (100203, 5 μg mL⁻¹), PE anti-mouse CD4 antibody [GK1.5] (100407, 2.5 μg mL⁻¹), and APC anti-mouse CD8a antibody [53-6.71] (100711, 2.5 μg mL⁻¹) were purchased from Biolegend.