Cell suspensions were collected after 2 days of culture and stained with combinations of the following Abs: APC anti-CD11b (eBioscience), AF700 anti-CD3e, AF750 anti CD4, PerCp-Cy5.5 anti-CD8a, BV421 anti-IgG, AF750 anti-CD19 (Biolegend), PE anti-CD138 (Syndecan-1, R&D Systems), FITC anti-IgA (Southern Biotech). For intracellular detection of IgG and IgA, golgi-stop was added to cell cultures 4 hours before harvest and extracellular staining with anti-CD19 and anti-CD138 Abs. Cells were then fixed and permeabilized before intracellular staining with anti-IgG and anti-IgA Abs. Stained cells were analyzed with an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA).
Af750 anti cd19
Manufactured by BioLegend
The AF750 anti-CD19 is a fluorescently labeled antibody that can be used to detect and quantify CD19-expressing cells in flow cytometry applications. CD19 is a cell surface protein expressed on B cells and B cell precursors.
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Lab products found in correlation
2 protocols using af750 anti cd19
1
Multiparametric Flow Cytometry for Immune Cell Analysis
2
Multiparameter Flow Cytometry of Mouse Splenic Immune Cells
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Mouse primary splenocyte suspension was collected and stained with combinations of the following antibodies: BV421 anti-IgG, AF750 anti-CD19 (Biolegend), PE anti-CD138 (Syndecan-1, R&D Systems), and FITC anti-IgA (Southern Biotech). For intracellular detection of IgG and IgA, Golgi-stop was added to cell cultures 4 h prior to harvest and extracellular staining was performed using anti-CD19 and anti-CD138 Abs. Cells were then fixed and permeabilized before intracellular staining with anti-IgG and anti-IgA Abs.Stained cells were analyzed on an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA).
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