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Mab5860

Manufactured by R&D Systems
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Sourced in United Kingdom

MAB5860 is a monoclonal antibody that recognizes human Somatostatin Receptor 2 (SSTR2). It can be used for the detection of SSTR2 expression in various applications such as flow cytometry, immunohistochemistry, and Western blotting.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Recombinant human april, by R&D Systems (2 mentions) Recombinant april, by Merck Group (2 mentions) Mab5931, by R&D Systems (2 mentions) A6730, by Merck Group (2 mentions) Pd98059, by Merck Group (1 mentions) Ly294002 hydrochloride, by Merck Group (1 mentions) Amd3100, by Merck Group (1 mentions) Human recombinant bdnf, by Alomone (1 mentions) Ly294002 hydrochloride l9908, by Merck Group (1 mentions) Pd98059 p215, by Merck Group (1 mentions)

3 protocols using mab5860

1

In Vitro Neuronal Transfection and APRIL Signaling

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After 2 or 6 days in vitro, the neurons were transfected with expression vectors using lipofectamine 2000 (Invitrogen, Paisley, UK) according to the manufacturer's instructions with modifications. Briefly, 1.2 μg of DNA was mixed with 4 μl of lipofectamine. After 20 min, this mixture was added to the cultures. After 3 h, the cultures were then washed with culture medium, at which point recombinant human APRIL was added (R&D Systems, Abingdon, UK) and the neurons were maintained in culture for further 18 h. Chemical inhibitors (PD98059, Sigma-P215, LY294002 hydrochloride, Sigma-L9908 and Akt1/2 kinase inhibitor A6730, Sigma-Aldrich Company, Dorset, UK) were added 2 h before incubation of recombinant APRIL. Function-blocking antibodies (mouse anti-BCMA, MAB5931 and human anti-APRIL, MAB5860, R&D Systems, Abingdon, UK) were added after transfection.
Osório C., Chacón P.J., White M., Kisiswa L., Wyatt S., Rodríguez-Tébar A, & Davies A.M. (2014). Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Molecular and Cellular Neurosciences, 59(100), 24-36.
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2

Modulation of Immune Cell Signaling

Cited 2 times
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Cultured cells were incubated with 100 ng/mL human recombinant CXCL1220 (link) (BioLegend, San Diego, CA, USA), 2 ng/mL human recombinant BAFF (BioLegend), and 25 ng/mL human recombinant APRIL (Peprotech, Neuilly-Sur-Seine, France) (these concentrations are comparable with the respective physiological levels in plasma from CLL patients21 (link),22 (link)) or 100 ng/mL human recombinant BDNF (Alomone Labs, Jerusalem, Israel), as described previously12 (link),23 (link). BAFF was neutralized using a goat anti-BAFF polyclonal antibody (20 ng/mL; #AF124, R&D Systems, Minneapolis, MN, USA) and APRIL was neutralized using a mouse anti-APRIL monoclonal antibody (1 μg/mL; #MAB5860, R&D Systems)10 (link). CXCR4 was inhibited using AMD3100 (0.5 μg/ml; Merck Millipore, Fontenay sous Bois, France) to reduce the CXCR4-positive cell population markedly24 (link). BDNF was neutralized using an anti-BDNF mouse monoclonal antibody (200 ng/mL; #GF35L, Merck Millipore).
Talbot H., Saada S., Barthout E., Gallet P.F., Gachard N., Abraham J., Jaccard A., Troutaud D., Lalloué F., Naves T., Fauchais A.L, & Jauberteau M.O. (2020). BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells. Scientific Reports, 10, 12572.
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3

Neuronal Transfection and APRIL Signaling

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After 2 or 6 days in vitro, the neurons were transfected with expression vectors using lipofectamine 2000 (Invitrogen, Paisley, UK) according to the manufacturer’s instructions with modifications. Briefly, 1.2 μg of DNA was mixed with 4 μl of lipofectamine. After 20 min, this mixture was added to the cultures. After 3 h, the cultures were then washed with culture medium, at which point recombinant human APRIL was added (R&D Systems, Abingdon, UK) and the neurons were maintained in culture for further 18 h. Chemical inhibitors (PD 98.059 P215, LY-294.002 hydrochloride L9908 and Akt1/2 kinase inhibitor A6730, Sigma-Aldrich Company, Dorset, UK) were added 2hr before incubation of recombinant APRIL. Function blocking antibodies (mouse anti-BCMA, MAB5931 and human anti-APRIL, MAB5860, R&D Systems, Abingdon, UK) were added after transfection.
Osório C., Chacón P.J., White M., Kisiswa L., Wyatt S., Rodríguez-Tébar A, & Davies A.M. (2014). Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Molecular and Cellular Neurosciences, 59(100), 24-36.
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