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Rhizomucor miehei

Manufactured by Merck Group
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Sourced in Germany

Rhizomucor miehei is a species of fungus commonly used in the production of lab equipment. It serves as a source of the enzyme lipase, which is used in various industrial and research applications. The core function of Rhizomucor miehei is the production of this enzyme.

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Lab products found in correlation

Rhizopus oryzae, by Merck Group (1 mentions) Rhizopus niveus, by Merck Group (1 mentions) Aspergillus niger, by Merck Group (1 mentions) Candida rugosa, by Merck Group (1 mentions)

3 protocols using rhizomucor miehei

1

Stereospecific Fatty Acid Analysis of Engineered Oilseeds

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Example 24

Stereospecific analysis of fatty acid species at sn-2 position of TAG from engineered seeds was conducted to assess the efficiency of assembly of short and medium chain fatty acids in TAG. Fatty acid profile of sn-2 monoacylglycerol obtained by digesting with TAG sn-1 and sn-3 specific lipase from Rhizomucor miehei (Sigma) revealed that while trace amounts of 10:0 is seen at sn-2 position of TAG from seeds of CvFatB1+CpDGAT1 lines, there is a striking increase up to 20 mol % in CvFatB1+CvLPAT2 line and 33.1 mol % in the relative content of 10:0 at sn-2 of TAG from CvFatB1+CvLPAT2+CpDGAT1 line was observed (FIG. 17). In addition to C10:0, 3 to 5 mol % of C8:0 was detected in TAG sn-2 position in the seeds engineered to express CvFatB1+CvLPAT2+CpDGAT1.

The increase of 10:0 at the sn-2 position of TAG from CvFatB1 expressing lines is accompanied by reduction of 18:2 and significantly that of 18:3 which is 17.4 mol % as compared to 44.3 mol % at this position in TAG from Wt camelina seeds. In C. pulcherrima fatty acid species at sn-2 position of TAG are 8:0 (up to 97 mol %) and C10:0 (3 mol %) while in C. viscosissima it is 12.6 mol % of C8:0 and 87.4 mol % of 10:0 (FIG. 17).

US10865421B2. Acyltransferases and methods of using (2020-12-15). NUtech Ventures [US]. Inventors: Edgar Cahoon [US], Umidjon Iskandarov [US], Hae Jin Kim [US], Jillian Collins-Silva [US].
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2

Hydrolysis of Diverse Vegetable Oils

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Hydrolysis reactions were performed by Aspergillus niger (product number 62301), Candida rugosa (product number 62316), Rhizopus oryzae (product number 62305), Rhizopus niveus (product number 62310) and Rhizomucor miehei (formerly known as Mucor miehei, product number 62298) free lipases obtained from Sigma–Aldrich (Munich, Germany). Seven oils, i.e., olive (refined; product number O1514), linseed (product number 430021), peanut (product number P2144), grape seed (product number W233218), rapeseed (product number 83450), almond (product number 63445) and menhaden fish (refined; product number F8020) oils, were involved to the assay. All oils were purchased from Sigma–Aldrich (Munich, Germany). PA (5.30–25.23%) was a major saturated fatty acid component in the oils used as determined by gas chromatography (GC) [46 (link)]. Concerning unsaturated fatty acids, OA (5.12–80.05%) and LA (1.91–23.79%) were among the major components of the oils. In addition, ALA (58.33%) in linseed oil and EPA (12.11%) and DHA (5.47%) in menhaden fish oil were also predominant fatty acid components. Individual fatty acids, i.e., EPA and ALA, were obtained from VWR (Debrecen, Hungary).
Kotogán A., Furka Z.T., Kovács T., Volford B., Papp D.A., Varga M., Huynh T., Szekeres A., Papp T., Vágvölgyi C., Mondal K.C., Kerekes E.B, & Takó M. (2022). Hydrolysis of Edible Oils by Fungal Lipases: An Effective Tool to Produce Bioactive Extracts with Antioxidant and Antimicrobial Potential. Foods, 11(12), 1711.
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3

Stereospecific Fatty Acid Analysis of Engineered Oilseeds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 24

Stereospecific analysis of fatty acid species at sn-2 position of TAG from engineered seeds was conducted to assess the efficiency of assembly of short and medium chain fatty acids in TAG. Fatty acid profile of sn-2 monoacylglycerol obtained by digesting with TAG sn-1 and sn-3 specific lipase from Rhizomucor miehei (Sigma) revealed that while trace amounts of 10:0 is seen at sn-2 position of TAG from seeds of CvFatB1+CpDGAT1 lines, there is a striking increase up to 20 mol % in CvFatB1+CvLPAT2 line and 33.1 mol % in the relative content of 10:0 at sn-2 of TAG from CvFatB1+CvLPAT2+CpDGAT1 line was observed (FIG. 17). In addition to C10:0, 3 to 5 mol % of C8:0 was detected in TAG sn-2 position in the seeds engineered to express CvFatB1+CvLPAT2+CpDGAT1.

The increase of 10:0 at the sn-2 position of TAG from CvFatB1 expressing lines is accompanied by reduction of 18:2 and significantly that of 18:3 which is 17.4 mol % as compared to 44.3 mol % at this position in TAG from Wt camelina seeds. In C. pulcherrima fatty acid species at sn-2 position of TAG are 8:0 (up to 97 mol %) and C10:0 (3 mol %) while in C. viscosissima it is 12.6 mol % of C8:0 and 87.4 mol % of 10:0 (FIG. 17).

US10280431B2. Acyltransferases and methods of using (2019-05-07). NUtech Ventures [US]. Inventors: Edgar Cahoon [US], Umidjon Iskandarov [US], Hae Jin Kim [US], Jillian Collins-Silva [US].
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