Human ifn γ elisa kit

Peripheral blood samples of 44 AS patients were available to detect T helper 1 (Th1) cell percentage and T Th17 cell percentage by flow cytometry using Human Th1/Th17 Phenotyping Kit (Thermo Fisher). Serum samples of all AS patients were applied to assess the level of interferon‐gamma (IFN‐γ) and interleukin 17A (IL‐17A) by enzyme‐linked immunosorbent assay (ELISA) using Human IFN‐γ ELISA Kit and Human IL‐17A ELISA Kit (Sangon Biotech), respectively. The experimentations were performed based on the manuals of manufacturers. The collected PBMC samples of all subjects (AS patients, OA patients, and HCs) were used to determine MALT1 expression by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR).
The total RNA was primarily fetched with RNeasy Protect Mini Kit (Qiagen). Besides, complementary DNA was then synthesized using PrimeScript™ RT reagent Kit (Takara). Meanwhile, qPCR was performed using KOD SYBR^® qPCR Mix (Toyobo). Moreover, Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was chosen to be the internal reference for MALT1, and the qPCR process was referred to preceding research.¹⁶ Then, the 2^−ΔΔCt method was applied to calculate MALT1 relative expression.

The levels of tumor necrosis factor α (TNF-α), interferon-gamma (IFN-γ), CXCL10, and CXCL9 in the co-culture system were detected according to the instructions of Human TNF-α ELISA Kit (D711045, Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., Shanghai, China), Human IFN-γ ELISA Kit (D711044, Sangon), Human CXCL10/IP-10 ELISA Kit (PC208, Beyotime), and MIG/CXCL9 Human ELISA Kit (EHCXCL9, Thermo Fisher).
The levels of TNF-α, IFN-γ, CXCL10, and CXCL9 in mouse xenograft tumors were detected according to the instructions of Mouse TNF-α ELISA Kit (PT512, Beyotime), Mouse IFN-γ ELISA Kit (PI508, Beyotime), Mouse CXCL10/IP-10 ELISA Kit (PC206, Beyotime), and MIG/CXCL9 Mouse ELISA Kit (EMCXCL9, Thermo Fisher).