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Uhr fe sem su 8010

Manufactured by Hitachi
Sourced in Japan

The UHR FE-SEM SU 8010 is a high-resolution field emission scanning electron microscope (FE-SEM) developed by Hitachi. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of applications.

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8 protocols using uhr fe sem su 8010

1

Morphology of Stomaphis quercus Labium and Stylets

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This study of labium and stylets in Stomaphisquercus was conducted on alcohol material of six adult specimens dehydrated in series of alcohol and dried in air. Additionally, one specimen from 70 % ethanol was dehydrated in series of alcohol and acetone and dried in the critical point (CPD), and one more specimen from 70 % ethanol was chemical dried in the hexamethyldisilazane (HMDS). All specimens were obtained from the collection of the Department of Zoology University of Silesia in Katowice.
The basal part of head with a part of the rostrum or the whole specimens was glued onto a scanning electron microscope stub. The materials used for SEM photographs were gold coated; the photographs were taken with a Hitachi UHR FE-SEM SU 8010 scanning electron microscope, with the samples placed in the high-pressure chamber.
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2

Multimodal Characterization of Materials

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Field emission scanning electron microscope (Fe-SEM) and energy-dispersive X-ray spectroscope (EDX) were carried out by Hitachi UHR Fe-SEM SU8010 with incident beam of 20 kV. X-ray diffraction (XRD) measurement was performed using benchtop X-ray powder diffractometer (Bruker) with Cu-Kα radiation (λ = 0.154184 nm). Micro-Raman measurement was conducted at room temperature using a 532 nm laser. The laser beam size is about 1 μm in diameter. X-ray photoelectron spectroscopy (XPS) was measured by Axis Supra, Kratos using 225 W Al-Kα monochromator. The base pressure was about 2.7 × 10−9 torr.
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3

Morphological Characterization of 3D Porous PU Scaffolds

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Before imaging, scaffold samples (~ 5 mm × 5 mm × 2 mm) were cut and placed onto a metallic stub by a double-sided carbon tape and coated with platinum using a Hitachi MC1000 Ion Sputter Coater for about 60 s. The morphology of 3D porous PU scaffold was observed by a Hitachi UHR FE-SEM SU8010 (Tokyo, Japan) at an acceleration voltage of 3 kV.
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4

SEM Analysis of RJFA Extract Morphology

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The surface morphology of RJFA extracts by UAE and SE method was attached to a stub with double-sided sticky tape and coated with gold before being examined by a HITACHI UHR FE-SEM SU8010 (HITACHI, Tokyo, Japan) under low vacuum mode at an accelerating voltage of 3.0 kV.
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5

Analyzing Pam. experimentalis thermal responses

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Active or tun-state Pam. experimentalis (n = 15 for each condition) were subjected to 20 °C, 35 °C, 37 °C, 40 °C, or 42 °C for 5 h. Then, the material was fixed with 2.5% glutaraldehyde, postfixed in 2% osmium tetroxide, dehydrated, and embedded according to the protocol described by Janelt et al.61 (link). Semi-thin (800 nm) and ultra-thin (70 nm) sections were cut using a Leica Ultracut EM UC7 ultramicrotome. Semi-thin sections were stained with 1% methylene blue in 1% borax and analyzed using an Olympus BX60 light microscope. Ultra-thin sections were mounted on copper grids and stained with uranyl acetate and lead citrate. Material was examined using a Hitachi H500 transmission electron microscope at 75 kV or a Hitachi UHR FE-SEM SU 8010 scanning electron microscope equipped with an ET (Everhart–Thornley) detector for imaging the sections at a low voltage of 25 kV.
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6

Scanning Electron Microscopy of Aphis fabae

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Forewings of three individuals of Aphis fabae species were examined using scanning electron microscopy. Samples were fixed and stored in 70% ethanol and then prepared using ethanol dehydration and hexamethyldisilazane (HMDS) drying. After 70% ethanol fixation, the material was dehydrated in a graded ethanol/water series of 75, 80, 90, 96, and 100% for 10 min in each concentration, and then there were three 100% ethanol changes. After dehydration, the samples were treated with HMDS 3 × 10 min and retained in HMDS after third change until the solution evaporated (Kanturski et al. 2015 (link)).
Samples were mounted on holders, sputter-coated with gold and examined using a scanning electron microscope (Hitachi UHR FE-SEM SU 8010, Tokyo, Japan) in the Scanning Electron Microscopy Laboratory at the Faculty of Biology and Environmental Protection, University of Silesia.
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7

Bacterial Biofilm Visualization on Nylon

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Imaging of the bacterial film present on the surface of the nylon monofilaments used in this study was performed with Hitachi UHR FE-SEM SU 8010 scanning electron microscope with an acceleration of 10 kV using a secondary electron detector (SED). Samples of a sterile nylon fibers and fibers immersed in 85 × 106 CFU/ml solution of B. subtilis were placed on aluminum stubs with adhesive carbon tape and allowed to dry completely at 22 °C for 12 h. After this time, the prepared material was covered with a 0.4 mm layer of gold in a Pelco SC-6 sputter coater (Ted Pella Inc., Redding, CA, USA) and observed in a scanning electron microscope.
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8

Scanning Electron Microscopy of Insect Antennae

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The material was obtained from the collections of the Natural History Museum in Vienna, Zoological Museum of the State Moscow University and Hungarian Natural History Museum in Budapest. Additional species of Nerthra and Gelastocoris have been donated by Ping-ping Chen. All specimens have been cleaned in an ultrasound cleaner, the antennae have been dissected, dried in ethanol, mounted, sputtered with gold or chromium and observed with the use of the scanning electron microscopes Phenom XL and Hitachi UHR FE-SEM SU 8010 in the scanning microscopy laboratory of the Faculty of Natural Science, Institute of Biology, Biotechnology and Environmental Protection of Silesian University in Katowice. We follow the terminology and classification reported in other papers on antennal sensilla of insects (Altner & Prillinger, 1980; Nowińska & Brożek, 2019) .
The antennae of eight species from three families have been studied:
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