Zetaview pmx 120 z nta instrument

Size distribution and concentration analyses of sEVs were performed using a ZetaView PMX 120-Z NTA instrument (Particle Metrix, Meerbusch, Germany) equipped with a CMOS camera and a 405-nm laser. Samples were diluted in filtered 1× PBS buffer to a concentration between 10⁷ and 10⁸ particles/ml and then injected into the instrument using a 1-ml syringe. Eleven positions with two readings per position were recorded for each sample. Data were analyzed using ZetaView software (Particle Metrix, Meerbusch, Germany), with hydrodynamic diameters of multiple particles being calculated via the Stokes-Einstein equation. The instrument’s default post-acquisition parameter settings with the 5-nm width of the bin class were applied.

Size distribution and concentration analyses of sEVs were performed using a ZetaView PMX 120-Z NTA instrument (Particle Metrix, Meerbusch, Germany) equipped with a CMOS camera and a 405-nm laser. Samples were diluted in filtered 1× PBS buffer to a concentration between 10⁷ and 10⁸ particles/ml and then injected into the instrument using a 1-ml syringe. Eleven positions with two readings per position were recorded for each sample. Data were analyzed using ZetaView software (Particle Metrix, Meerbusch, Germany), with hydrodynamic diameters of multiple particles being calculated via the Stokes-Einstein equation. The instrument’s default post-acquisition parameter settings with the 5-nm width of the bin class were applied.