176 blood samples were collected from healthy blood donors in the Blood Center of Zhejiang province, China. The ethnic background of all individuals is Zhejiang Han. Informed consent was obtained from all participants. The Zhejiang Han are a population of approximately 60 million individuals living on the Southeast coast of China and are a subgroup of the Chinese Southern Han ethnic group. This study was approved by the regional ethics committee, Blood Center of Zhejiang Province. Genomic DNA was extracted using MagNA Pure LC DNA Isolation Kits (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturer’s instructions. The final DNA concentration was adjusted to 60 ng/μl and optical density at 260/280 was approximately 1.8.
Magna pure lc dnaisolation kits
Manufactured by Roche
Sourced in United States
The MagNA Pure LC DNA Isolation Kits are a set of laboratory equipment designed for the automated extraction and purification of DNA from various sample types. The kits utilize magnetic bead-based technology to efficiently isolate high-quality DNA for downstream applications.
Automatically generated - may contain errors
4 protocols using magna pure lc dnaisolation kits
1
Zhejiang Han DNA Extraction Protocol
2
Genotyping of HBV-related SNPs
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Four genetic variants (rs477515, rs9276370, rs7756516, rs9277535) associated with persistent HBV infection that were previously identified[17 (link)] were included in the analysis. One additional HCC-related SNP (rs9272105) previously identified in China was also included[26 (link)]. Genomic DNA was extracted from peripheral blood cells using MagNA Pure LC DNA isolation kits with automated DNA isolation instruments (MagNA Pure LC II; Roche Diagnostics, Mannheim, Germany). Triple-SNP (rs477515, rs9272105, rs9277535) genotyping was performed with TaqMan Genotyping assays (Applied Biosystems, Foster City, CA, United States). Two SNPs (rs7756516, rs9276370) were genotyped with a Sequenom MassARRAY System (Sequenom, San Diego, CA, United States). The TaqMan assays were carried out by Vita Genomics (New Taipei City, Taiwan), and the Sequenom MassARRAY assays were performed by the Academia Sinica National Genotyping Center (Taipei, Taiwan). The overall genotype call rate was > 95%.
3
DNA Extraction Using MagNA Pure LC
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Genomic were extracted using commercial MagNA Pure LC DNAIsolation Kits (Roche Diagnostics, Indianapolis, IN, USA) accordingto the manufacturer's instruction. The nally DNA concentrationwas adjusted in 15-30 ng/µl and optical density at 260/280 was1.6-1.8.
4
DNA Extraction Using MagNA Pure LC
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Genomic were extracted using commercial MagNA Pure LC DNAIsolation Kits (Roche Diagnostics, Indianapolis, IN, USA) accordingto the manufacturer's instruction. The nally DNA concentrationwas adjusted in 15-30 ng/µl and optical density at 260/280 was1.6-1.8.
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