Cd117 2b8

Manufactured by Thermo Fisher Scientific

The CD117 (2B8) is a lab equipment product manufactured by Thermo Fisher Scientific. It is an antibody designed for the identification and characterization of cells expressing the CD117 antigen, also known as c-kit. The CD117 (2B8) can be used in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using cd117 2b8

Multiparameter Flow Cytometry Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stained cells were analyzed using a BD LSRII™ or a FACSymphony™ flow cytometer and a FACSDiva 9.1 software (BD). Cell viability was evaluated using SYTOX™ Blue Dead Cell Stain (Invitrogen) or DAPI (Merck). The following antibodies were used. CD4 (RMA‐5); CD8α (53–6.7), CD5 (53–7.3), CD16/CD32 (2.4G2), CD19 (1D3), CD24 (M1/69), CD25 (PC61), CD27 (LG.3A10), CD34 (RAM34), CD43 (S7), CD44 (IM7), CD45.1(A20), CD45.2 (104), CD45R (RA3‐6B2), CD62L (Mel‐14), BP‐1 (6C3), IgM (R6‐60.2), IgD (11‐26c.2a), Ly‐6G (clone 1A8), Ly‐6C (AL‐21), Sca‐1 (Ly‐6A/E, D7), TCRβ (H57‐597), TCRγδ (GL3), Ter119 (TER‐119), all from BD Biosciences. CD11b (M1/70), CD3 (17A2), CD150 (TC15‐12F12.2), CD135 (A2F10), CD161c (PK136), CD11c (N418), CD45 (30‐F11), CD19 (6D5), and CD197 (CCR7, 4B12) all from BioLegend, CD127 (A7R34) and CD117 (2B8) both from eBioscience, and LFA‐1 (CD11a, 121/7) from Invitrogen.

He L., Valignat M., Zhang L., Gelard L., Zhang F., Le Guen V., Audebert S., Camoin L., Fossum E., Bogen B., Wang H., Henri S., Roncagalli R., Theodoly O., Liang Y., Malissen M, & Malissen B. (2021). ARHGAP45 controls naïve T‐ and B‐cell entry into lymph nodes and T‐cell progenitor thymus seeding. EMBO Reports, 22(4), e52196.

+ Open protocol

+ Expand

Multiparameter Analysis of Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bones, spleen, and thymus were dissected, crushed in PBS with 2% FCS and cells were collected after passing through a 70 μm filter. They were then Fc-blocked (CD16/32; 93) and stained with combinations of the antibodies Sca1 (D7), CD105 (MJ7/18), CD41 (MWReg30), CD48 (HM48-1), CD3 (145-2C11), CD4 (RM4-5), CD8 (53-6.7), B220 (RA3-6B2), NK1.1 (PK136), Mac1 (M1/70), Gr1 (RB6-8C5), TER119 (TER-119), CD150 (TCF15-12F12.2), CD117 (2B8, eBioscience), CD127 (A7R34), CD44 (IM7), CD25 (PC61.5, eBioscience), CD19 (1D3, eBioscience), TcRβ (H57-597, eBioscience), TcRγδ (GL3, eBioscience), Ly6C (AL-21), Ly6G (1A8), MHCII (M5/114.15.2), CD11c (N418), PDCA1 (927), Ly6D (49H4), Flt3 (A2F10), IgD (11-26c.2a), and IgM (11/41, eBioscience). All antibodies were purchased from BD Biosciences unless otherwise indicated. Propidium iodide (PI) was utilized to discriminate dead cells. For hematopoietic stem and progenitor cell isolation, cells were subjected to lineage depletion using Dynabeads sheep anti rat IgG (Life Technologies) together with TER119, CD19, CD3, Gr1, and CD11b antibodies prior to staining. Analysis and cell sorting was performed primarily on an LSR Fortessa and FACSAria IIu (BD Biosciences). Analysis of data was done using the Flowjo 9.9.6 software (Flowjo).

Bouderlique T., Peña-Pérez L., Kharazi S., Hils M., Li X., Krstic A., De Paepe A., Schachtrup C., Gustafsson C., Holmberg D., Schachtrup K, & Månsson R. (2019). The Concerted Action of E2-2 and HEB Is Critical for Early Lymphoid Specification. Frontiers in Immunology, 10, 455.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!