Antibody against caspase 1

Immunohistochemistry (IHC) was used to detect the immunoreactivity of caspase-1. Serial slides were rinsed in PBS and blocked in 5% BSA for 1 h, then reacted with an antibody against caspase-1 (1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 2 h. The sections were then incubated with a secondary antibody labeled with horseradish peroxidase for 30 min at room temperature. Cells with brownish-yellow cytoplasm were counted as positive cells. For quantitative immunostaining, caspase-1-positive cells were observed under an inverted microscope, and the CA1 region was counted for all groups in ImageJ software.

Quercetin (HPLC purity ≥ 95%), phorbol 12-myristate 13-acetate (PMA), dimethyl sulfoxide (DMSO; ≥ 99.5%) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against IL-1β, NLRP3 (D4D8T), p44/42 MAPK (Erk1/2)(137F5), phospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204), SAPK/JNK, phospho-SAPK/JNK (Thr183/Tyr185), p38 MAPK and phospho-p38 MAP Kinase (Thr180/Tyr182) were from Cell Signaling Technology (USA). Antibody against caspase-1 was purchased from Santa Cruz Biotechnology (USA) and β-actin antibody was from Abcam (USA). Peroxidase-conjugated AffiniPure goat anti-mouse IgG and anti-rabbit IgG, HRP-linked antibody as secondary antibodies were from Jackson ImmunoResearch Laboratories (USA) and Cell Signaling Technology, respectively.