Markers of apoptosis (p38 mitogen-activated protein kinase (p38 MAPK) and caspase 3) were measured by p38 MAPK alpha ELISA Kit (Abcam, Cambridge, UK) and Rabbit Caspase 3 ELISA Kit (Cusabio, Wuhan, China) according to the manufacturer’s instructions. Data were expressed as ng/mL of tissue homogenate.
Rabbit caspase 3 elisa kit
Manufactured by Cusabio
Sourced in United Kingdom
The Rabbit Caspase 3 ELISA Kit is a quantitative test kit designed to measure the levels of Caspase 3 in rabbit samples. Caspase 3 is a key enzyme involved in the apoptosis (programmed cell death) pathway. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify Caspase 3 concentrations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit caspase 3 elisa kit
1
Measuring Apoptosis Markers in Tissue
2
Lung Tissue Biochemical Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For biochemical evaluations, strips of the right lung tissue were homogenized in ice-cold PBS (0.02 mol/l, pH 7.2) for final concentration 10 % (weight/volume). Homogenates were subjected to two freeze-thaw cycles and centrifuged for 15 min at 1500 g. Then, supernatants were removed and analyzed.
For cytokine evaluation, ELISA kits for Rabbit IL-1β and IL-6 (USCN Life Science, Wuhan, China) were used according to the manufacturer's instructions. Data were expressed as pg/ml of homogenate.
Apoptotic markers were evaluated by p38 MAPK alpha ELISA Kit (Abcam, Cambridge, UK) and Rabbit Caspase 3 ELISA Kit (Cusabio, Wuhan, China) according to the manufacturer's instructions. Data were expressed as ng/ml of homogenate.
For evaluation of oxidative markers, OxiSelect TBARS Assay Kit (Cell Biolabs Inc., San Diego, USA) was used for malondyaldehyde/thiobarbituric reactive substances (TBARS; product of lipid peroxidation). Data were expressed as μmol/ml of homogenate. Total antioxidant capacity (TAC) was assessed using TAC assay (Abcam, Cambridge, UK) and data were expressed as Trolox equivalent per ml of homogenate.
For cytokine evaluation, ELISA kits for Rabbit IL-1β and IL-6 (USCN Life Science, Wuhan, China) were used according to the manufacturer's instructions. Data were expressed as pg/ml of homogenate.
Apoptotic markers were evaluated by p38 MAPK alpha ELISA Kit (Abcam, Cambridge, UK) and Rabbit Caspase 3 ELISA Kit (Cusabio, Wuhan, China) according to the manufacturer's instructions. Data were expressed as ng/ml of homogenate.
For evaluation of oxidative markers, OxiSelect TBARS Assay Kit (Cell Biolabs Inc., San Diego, USA) was used for malondyaldehyde/thiobarbituric reactive substances (TBARS; product of lipid peroxidation). Data were expressed as μmol/ml of homogenate. Total antioxidant capacity (TAC) was assessed using TAC assay (Abcam, Cambridge, UK) and data were expressed as Trolox equivalent per ml of homogenate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!