AFM was accomplished using a Multimode Nanoscope V system (Bruker, Santa Barbara, CA). Tapping mode AFM was used to map the topography by tapping the surface using an oscillating tip. The measurements were achieved using commercial Si cantilevers with a nominal spring constant and resonance frequency at 20–80 Nm−1 and 230–410 kHz, respectively (TESP, Bruker AFM Probes, Santa Barbara, CA). AFM samples were prepared by dropping casting onto oxidized silicon silicon wafer (100 nm thick thermal oxide) and then dried at room temperature before observation.50 The silicon wafers were cleaned using acetone-water mixture and then isopropyl alcohol or ethanol. Nanoparticle sample solutions were diluted with deionized water to a concentration of 0.20% w/w.
Multimode nanoscope 5 system
Manufactured by Bruker
The Multimode Nanoscope V system is a high-performance atomic force microscope (AFM) designed for advanced nanoscale characterization. It provides precise topographical and material property measurements on a wide range of samples. The system features a modular design, allowing for the integration of multiple imaging and spectroscopy modes to meet the needs of various research and industrial applications.
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Lab products found in correlation
2 protocols using multimode nanoscope 5 system
1
Topographical Mapping of Nanoparticles using AFM
2
Atomic Force Microscopy of PEG Samples
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Samples for AFM analysis were prepared by dissolving PEG in Milli-Q water to a concentration of 3% (w/v). A drop of PEG solution was placed on a polystyrene Petri dish (Iwaki, 1000–035, non-treated) for 1 h at 37°C. Then the solution was removed, and Milli-Q water was added. Hydrated samples were then mounted into the AFM liquid cell. AFM measurements were carried out using a Multimode/Nanoscope V system (Bruker). AFM images were acquired in contact mode in liquid (MilliQ water) using commercial Si3N4 cantilevers (DNP-10 Bruker, k = 0.24 N/m). For some samples PEG solution was not removed and AFM measurements were performed in PEG solution. No significant differences were observed between images acquired in Milli-Q water or PEG solution. Data were analyzed with Gwyddion software.
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