Goat anti mouse igg fitc conjugates

RADA16-I peptide was commercially synthesized by Fmoc solid phase method and purchased from BD Biosciences (catalog No.354250, Shanghai, China), which had 16 amin acid residues sequence Ac-RADARADARADARADA-CONH₂ (RADA16-I in short). The lyophilized powder was prepared at a concentration of 10 mg % (w/v) in sterile ultrapure water (18.2 MΩ, Millipore, Bedford, USA) and sonicated 30 min and then stored at 4 °C for use. Collagen I (catalog No. 354236, rat tail) and Matrigel (catalog No. 356234, growth factors reduced from mouse EHS sarcoma) were purchased from BD Biosciences and used for 3D cell culture assay. Sucrose, 4′6-diamidino-2-phenylindole (DAPI), Trypsin–EDTA, calcein-AM, phalloidin, BrdU, anti-BrdU monoclonal antibody (catalog No. B2531) and goat anti-mouse IgG-FITC conjugates (catalog No. BA1101) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640 and fetal bovine serum (FBS) were obtained from Gibco Life Technology Inc. (GIBCO, Invitrogen, Carlsbad, CA, USA). E-cadherin (ab76055), N-cadherin (ab18203) and integrin β₁ (ab179471) antibodies were obtained from Abcam (Cambridge, UK). Other assay reagents were mainly obtained from Gibco/Thermo Fisher Scientific (Rockville, MD, USA). The cell images were taken with an inverted Olympus IX71 fluorescent microscope and Leica DMILLED microscope.

Recombinant yeast was collected and subjected to immunofluorescence assay following the procedure described elsewhere [37 (link)]. Mouse anti-HA antibodies (1 : 500 dilution) and goat anti-mouse IgG-FITC conjugates (Sigma) (1 : 5000 dilution) were used.