E cadherin rabbit antibody

Cells were seeded on the coverslips and cultured for proper cell density. The cells were blocked with FBS after fixing by 4% paraformaldehyde (Aladdin, China). Then, the cells were incubated with the E-cadherin rabbit antibody (proteintech, China) overnight at room temperature. Next, these samples were washed with PBS and incubated with fluorescein Cy3-conjugated secondary antibodies (Beyotime, China). Then, the samples were counterstained with DAPI (Aladdin, China). Images were captured using the fluorescence microscope (Olympus, Japan).

Cells were lysed in RIPA buffer (Solarbio, China). BCA protein assay kit (Solarbio, China) was used to assay the protein concentrations. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Solarbio, China) was used to separate proteins. Then, these proteins were transferred onto polyvinylidene fluoride membrane (Millipore, USA). After blocking with 5% BSA (Biosharp, China) in PBST (PBS with 0.05% Tween-20), membranes were incubated with E-cadherin rabbit antibody (Proteintech, China), N-cadherin rabbit antibody (Proteintech, China), LIVIN rabbit antibody (Proteintech, China), p-IκB rabbit antibody (Affinity, China), IκB rabbit antibody (Affinity, China), p-P65 rabbit antibody (Affinity, China), P65 rabbit antibody (Affinity, China) and GAPDH mouse antibody (Proteintech, China) overnight at 4°C, respectively. Then, these membranes were incubated with goat anti-rabbit IgG secondary antibody (Solarbio, China) and goat anti-mouse IgG secondary antibody (Solarbio, China) at 37°C for 1 h. The antigen-antibody reaction was observed by the ECL assay (Solarbio, China).