Islets were fixed in 2% (wt/vol) glutaraldehyde (Sigma-Aldrich) and then submitted to the Electron Microscopy Facility at McMaster University (Canada). Islets were post-fixed with 1% (wt/vol) osmium tetroxide, dehydrated in ethanol, embedded in Spurr’s resin and sectioned with a Leica UCT ultramicrotome. Sections were stained with uranyl acetate and lead citrate and imaged with a JEM 1200 EX TEMSCAN transmission electron microscope (JEOL, USA). Insulin granules were quantified using ImageJ v1.52a [49 (link)].
Jem 1200 ex temscan transmission electron microscope
Manufactured by JEOL
Sourced in United States
The JEM 1200 EX TEMSCAN is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging of small-scale structures and materials. The core function of the JEM 1200 EX TEMSCAN is to magnify and project an image of a specimen onto a fluorescent screen or a digital detector, allowing for detailed observation and analysis of the sample's internal structure and composition at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using jem 1200 ex temscan transmission electron microscope
1
Ultrastructural Analysis of Pancreatic Islets
2
Biofilm Ultrastructural Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biofilm cells were aseptically scraped from flow cells, and the material was transferred directly to sterile microcentrifuge tubes. The tubes were couriered on ice to the Electron Microscope Facility, McMaster University, ON, Canada, where further processing of the samples was immediately performed, as per the procedure followed by Lawrence et al. (74 (link)), with a few modifications. Ultrathin sections were cut with a diamond knife mounted on a Leica UCT Ultramicrotome and placed on TEM grids (Marivac Canada, Canton de Gore, Quebec, Canada). Lastly, sample thin sections were poststained with uranyl acetate, followed by Reynold’s lead citrate staining, and then viewed on a JEOL JEM 1200 EX TEMSCAN transmission electron microscope (JEOL, Peabody, MA).
3
Hydrogel Structure Analysis via TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hydrogel samples were prepared as described above and submerged in 10 mM PBS to swell for at least 24 h. A slow solvent exchange to ethanol was performed to minimize the collapse of pore structure, using increasing ethanol solutions of 0, 10, 20, 30, 40, 50, 75, 95 and 100 vol %. Pieces of hydrogel samples were then quick frozen in liquid nitrogen and placed into a pre-cooled (-145°C) FC 4E cryochamber attached to an Ultracut E ultramicrotome (Reichert-Jung Wien, Austria). Thin sections (unstained) were cut with a diamond knife and placed onto Formvar-coated Cu grids which were allowed to warm to room temperature prior to imaging using a JEOL JEM 1200 EX TEMSCAN transmission electron microscope (JEOL, Peabody,
MA) operating at an accelerating voltage of 80 kV.
MA) operating at an accelerating voltage of 80 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!