S. Typhimurium cultures were grown overnight in LB at 37°C with aeration. Cultures were diluted to ~5 X 106 bacteria/ml into M9 or LB. For the growth curve experiments, culture volumes of 180 μl were added to a flat-bottomed 96-well plate. Capecitabine was freshly diluted in water, and 20 μl volumes were added to reach the final concentrations indicated. Plates were grown for 24 h at 37°C with shaking, and the OD600 was measured hourly in a Synergy 2 multi-mode or Eon microplate reader (BioTek, Winooski, VT). Mean OD600 and SEM from four biological replicates tested in triplicate were calculated. For the endpoint analysis experiments, S. Typhimurium cultures were grown overnight at 37°C in LB with aeration. Cultures were diluted 100-fold to approximately 5 X 106 CFU/ml in M9 or in LB. Diluted culture (90 μl) was added to a 96-well round-bottomed plate. Capecitabine was freshly diluted in 50% DMSO and 10 μl was added to culture wells to reach the final concentrations indicated with 5% DMSO. Plates were grown statically for 24h at 37°C, and the OD620 was measured with an accuSkan FC microplate photometer (ThermoFisher Scientific, Waltham, MA).
Accuskan fc microplate photometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The AccuSkan FC microplate photometer is a compact and versatile laboratory instrument designed for absorbance-based assays. It can be used to measure the optical density of samples in microplates, providing accurate and reliable results for a variety of applications. The core function of the AccuSkan FC is to quantify the amount of light absorbed by a sample, which is directly related to the concentration of the analyte of interest.
Automatically generated - may contain errors
4 protocols using accuskan fc microplate photometer
1
Evaluating Antibiotic Effects on Salmonella Growth
2
Assay for Salmonella Growth Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
S. Typhimurium cultures were grown overnight in LB at 37°C with aeration. Cultures were diluted to ∼5 × 106 bacteria/ml into M9 or LB. For the growth curve experiments, culture volumes of 180 μl were added to a flat-bottomed 96-well plate. Capecitabine was freshly diluted in water, and 20 μl volumes were added to reach the final concentrations indicated. Plates were grown for 24 h at 37°C with shaking, and the OD600 was measured hourly in a Synergy 2 multi-mode or Eon microplate reader (BioTek, Winooski, VT). Mean OD600 and SEM from four biological replicates tested in triplicate were calculated. For the endpoint analysis experiments, S. Typhimurium cultures were grown overnight at 37°C in LB with aeration. Cultures were diluted 100-fold to approximately 5 × 106 CFU/ml in M9 or in LB. Diluted culture (90 μl) was added to a 96-well round-bottomed plate. Capecitabine was freshly diluted in 50% DMSO and 10 μl was added to culture wells to reach the final concentrations indicated with 5% DMSO. Plates were grown statically for 24 h at 37°C, and the OD620 was measured with an accuSkan FC microplate photometer (ThermoFisher Scientific, Waltham, MA).
3
Assay for Salmonella Growth Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
S. Typhimurium cultures were grown overnight in LB at 37°C with aeration. Cultures were diluted to ∼5 × 106 bacteria/ml into M9 or LB. For the growth curve experiments, culture volumes of 180 μl were added to a flat-bottomed 96-well plate. Capecitabine was freshly diluted in water, and 20 μl volumes were added to reach the final concentrations indicated. Plates were grown for 24 h at 37°C with shaking, and the OD600 was measured hourly in a Synergy 2 multi-mode or Eon microplate reader (BioTek, Winooski, VT). Mean OD600 and SEM from four biological replicates tested in triplicate were calculated. For the endpoint analysis experiments, S. Typhimurium cultures were grown overnight at 37°C in LB with aeration. Cultures were diluted 100-fold to approximately 5 × 106 CFU/ml in M9 or in LB. Diluted culture (90 μl) was added to a 96-well round-bottomed plate. Capecitabine was freshly diluted in 50% DMSO and 10 μl was added to culture wells to reach the final concentrations indicated with 5% DMSO. Plates were grown statically for 24 h at 37°C, and the OD620 was measured with an accuSkan FC microplate photometer (ThermoFisher Scientific, Waltham, MA).
4
Quantification of Murine Mucins and IL-13
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ELISAs for murine MUC5AC (M7906) and murine MUC5B (M7978) were purchased from Biotang (Lexington, MA, US). BAL samples were run in duplicate and read using a filter-based accuSkan FC microplate photometer (ThermoFisher Scientific, Waltham, MA, US). Concentrations were determined by an 8-point standard curve ranging from 0.625 to 80 ng/mL plotted in a 4-parameter logistic sigmoidal curve (R2 > 0.99). MUC5AC and MUC5B protein standards were provided in the kits. The limits of sensitivity were 0.3 ng/mL for both. The intra-assay coefficients of variability were 8.89% and 6.88% for MUC5AC and MUC5B, respectively. An ELISA for IL-13 (M1300CB) was purchased from R&D Systems (Minneapolis, MN). Concentrations were determined by an 8-point standard curve with ranges of 7.8–250 pg/mL and plotted in a 4-parameter logistic sigmoidal curve (R2 > 0.99). The intra-assay and inter-assay variabilities were 2.8% and 3.6%, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!