A Cytokine and Chemokine 26-Plex Mouse ProcartaPlexTM Panel 1 (Thermofischer Scientific, Waltham, MA, USA) was used to assess cytokine levels in mouse plasma samples, according to the manufacturer’s instructions. We used a Luminex MAGPIX instrument (Luminex Corporation, Northbrook, IL, USA) calibrated with MAGPIX Calibration and Performance Verification Kits (Millipore, Burlington, MA, USA) with xPONENT software (Luminex) to obtain data. Data were analysed using Multiplex Analyst software version 5.1 (Merck, Darmstadt, Germany) as the Median Fluorescent Intensity (MFI) using spline curve-fitting for calculating analyte concentrations in samples.
Multiplex analyst software version 5
Manufactured by Merck Group
Sourced in Germany
Multiplex Analyst software version 5.1 is a data analysis tool designed to process and analyze data generated by Merck's multiplex assay platforms. The software provides functionalities for data visualization, statistical analysis, and report generation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using multiplex analyst software version 5
1
Mouse Cytokine and Chemokine Profiling
2
Multiplex Cytokine Profiling in Serum
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A 34-plex human cytokine/chemokine panel (ProcartaPlex; Thermo Fisher Scientific) was used to assess chemokine and cytokine levels in human serum according to the manufacturer's instructions. Luminex MAGPIX instrument (Luminex Corporation) calibrated with MAGPIX Calibration and Performance Verification Kits (Millipore) with xPONENT software (Luminex) was used to acquire data. Saved data were then analysed with Multiplex Analyst software version 5.1 (Merck) as the median fluorescent intensity (MFI) using spline curvefitting for calculating analyte concentrations in samples. Cytokines were included in the analysis if they were detectable in a minimum of 30% of participants, 30% of the time (Figure 2).
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