Polycystin 1

Manufactured by Santa Cruz Biotechnology

Polycystin‐1 is a protein that functions as a mechanosensitive calcium channel in the primary cilium of various cell types. It plays a role in sensing fluid flow and mediating cellular responses to mechanical stimuli.

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Lab products found in correlation

Phospho erk1 2, by Abcam (1 mentions) Phospho mtor, by Santa Cruz Biotechnology (1 mentions) Polycystin 2, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat igg hrp conjugate, by GenScript (1 mentions) Erk1 2, by Santa Cruz Biotechnology (1 mentions) Phospho fak, by Santa Cruz Biotechnology (1 mentions) Laminin, by Merck Group (1 mentions) B 1035, by Vector Laboratories (1 mentions) Sp 2001, by Vector Laboratories (1 mentions) Megalin, by Santa Cruz Biotechnology (1 mentions) Cubilin, by Santa Cruz Biotechnology (1 mentions) Villin 1, by Cell Signaling Technology (1 mentions) B 1325, by Vector Laboratories (1 mentions)

2 protocols using polycystin 1

Western Blot Analysis of Key Signaling Proteins

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The following primary antibodies were used for Western blot analysis: polycystin‐1 (sc‐130554, Santa Cruz Biotechnology), polycystin‐2 (sc‐10376, Santa Cruz Biotechnology), ERK1/2 (sc‐514302, Santa Cruz Biotechnology), phospho‐ERK1/2 (Abcam ab32538), mTOR (701483, Thermo Fisher Scientific), phospho‐mTOR (5536 CST), FAK (sc‐271126, Santa Cruz Biotechnology), phospho‐FAK (8556 CST), YAP (sc‐101199, Santa Cruz Biotechnology), TAZ (sc‐518026, Santa Cruz Biotechnology) and actin (MAB1501, Millipore). The following secondary antibodies were used: goat anti‐mouse IgG HRP‐conjugate (AP124P, Millipore), goat anti‐rabbit IgG HRP‐conjugate (AP132P, Millipore) and donkey anti‐goat IgG HRP‐conjugate (A00178, GenScript).

Zoi I., Gargalionis A.N., Papavassiliou K.A., Nasiri‐Ansari N., Piperi C., Basdra E.K, & Papavassiliou A.G. (2022). Polycystin‐1 and hydrostatic pressure are implicated in glioblastoma pathogenesis in vitro. Journal of Cellular and Molecular Medicine, 26(5), 1699-1709.

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Comprehensive Kidney Tissue Imaging Protocols

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For immunohistochemistry (IHC), kidney sections were pretreated to quench endogenous peroxidase (3.0% hydrogen peroxide) and endogenous biotin (SP-2001, Vector Labs), and stained with biotinylated LTL (B-1325, Vector Labs) and DBA (B-1035, Vector Labs) following standard IHC protocol. For immunofluorescence staining, mouse kidneys were fixed in 4% PFA followed by incubation in 30% sucrose overnight at 4°C, embedded in OCT compound (Tissue-Tek), and cryosectioned at 10 μm. Frozen sections were permeabilized with 0.1% PBS- Triton X-100 for 10 min and blocked in 5% goat serum for 1 hour. Primary antibodies were incubated overnight at 4°C followed by secondary antibodies incubated at room temperature for 1 hour. Following primary antibodies were used: WT1 (sc-192, Santa Cruz), Polycystin-1 (sc-130554, Santa Cruz), Laminin (L9393, Sigma), GFP (GFP-1020, Aves), Villin-1 (2369, Cell Signaling), JAG1 (sc-8303, Santa Cruz), PAX2 (71-6000, Thermo Fisher), megalin (sc-16478, Santa Cruz), cubilin (sc-20609, Santa Cruz). Tissue sections were mounted in media containing DAPI and imaged by a Zeiss confocal microscope.

Rasouly H.M., Kumar S., Chan S., Pisarek-Horowitz A., Sharma R., Xi Q.J., Nishizaki Y., Higashi Y., Salant D.J., Maas R.L, & Lu W. (2016). Loss of Zeb2 in mesenchyme-derived nephrons causes primary glomerulocystic disease. Kidney international, 90(6), 1262-1273.

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