Dry strip cover fluid

IPG strips pH 3⿿10 NL and dry strip cover fluid were purchased from GE Health Care Europe (Uppsala, Sweden). The ECL detection system was purchased from PerkinElmer (MA, USA). Anti-S100A11 (calgizzarin), anti-serum amyloid A1(SAA1) and anti-chloride intracellular channel protein 3 (CLIC3) specific primary antibodies were from Abcam, (Cambridge, UK). Anti-γ enolase (ENO2), was from Cell Signaling Technology (MA, USA). Goat anti-rabbit IgG-HRP conjugate secondary antibody was from stress gene. All other reagents were acquired from standard commercial sources and were of the highest grade available.

Immobilized pH gradient strip (pH 3–10), pharmalytes, drystrip cover fluid, bromophenol blue, agarose, Cy2, Cy3, and Cy5 were purchased from GE Healthcare (Little Chalfont, UK). Protease inhibitor cocktail (Pefabloc SC and Pefabloc SC protector) was purchased from Roche (Mannheim, Germany). Sequence-grade trypsin was obtained from Promega UK (Southampton, Hants, UK). All other chemicals used in this study were of the highest grade available and were purchased from GE Healthcare, Dojin Chemical Japan (Osaka, Japan), Sigma (St. Louis, MO), Wako (Osaka, Japan), Nacalai Tesque (Kyoto, Japan), or Kanto (Tokyo, Japan). The crude herbal drug SJG was prepared by Wakanyaku Medical Institute, Ltd. (Maebashi, Japan). SJG is composed of a water extract of kumazasa leaves and ethanol extracts of Japanese red pine leaves and ginseng roots in the ratio 8:1:1 [14] (link). SJG was supplied as a liquid preparation and diluted using tap water to 50% (v/v). We previously analyzed the component of SJG and found that SJG contained several compounds like tricin, p-coumaric acid, ginsenoside [8] .