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Anti hmga2

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Anti-HMGA2 is a laboratory reagent used for the detection and quantification of HMGA2 protein in various biological samples. It is a specific antibody that binds to the HMGA2 protein, allowing for its identification and measurement through techniques such as Western blotting, immunohistochemistry, or ELISA.

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Lab products found in correlation

Anti actin, by Merck Group (2 mentions) Anti hras, by Santa Cruz Biotechnology (2 mentions) Anti e1a, by Santa Cruz Biotechnology (2 mentions) Anti cyclin a2, by Merck Group (2 mentions) Anti histone h3, by Abcam (1 mentions) Mouse anti p21, by Santa Cruz Biotechnology (1 mentions) Anti srebp 1, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Abcam (1 mentions) Anti human p21, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Anti hmga2, by GeneTex (1 mentions) Anti α actin, by Santa Cruz Biotechnology (1 mentions) Anti p53, by Merck Group (1 mentions) Anti parp, by Cell Signaling Technology (1 mentions)

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HMGA2 (9 citations)

3 protocols using anti hmga2

1

Immunoblotting analysis of key proteins

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The following antibodies were used for immunoblotting: anti-HRAS (Santa Cruz, sc-29), anti-human p21 (Santa Cruz, sc-397), anti-E1A (Santa Cruz, sc-430); anti-ß-actin (Sigma A5441), anti-Cyclin A2 (Sigma C4710), anti-human p53 (DO-1, Sigma P6874), anti-MDM2 (clones 2A10 and 4B11) [18 (link)], anti-Histone H3 (Abcam ab1791), anti-HMGA2 (Santa Cruz, sc-30223), anti-SCD/Scd1 (Cell Signaling, #2438), anti-mouse p53 (Biovision #3036) and anti-mouse p21 (Santa Cruz #sc-6246), anti-α-Tubulin (Abcam #Ab18251), anti-SREBP1 (Santa Cruz, sc-13551). Immunoblotting analysis was carried out as described [18 (link)].
Kirschner K., Samarajiwa S.A., Cairns J.M., Menon S., Pérez-Mancera P.A., Tomimatsu K., Bermejo-Rodriguez C., Ito Y., Chandra T., Narita M., Lyons S.K., Lynch A.G., Kimura H., Ohbayashi T., Tavaré S, & Narita M. (2015). Phenotype Specific Analyses Reveal Distinct Regulatory Mechanism for Chronically Activated p53. PLoS Genetics, 11(3), e1005053.
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2

Western Blot Analysis of HMGA Proteins

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The single-cell suspension was obtained by mechanical disaggregation of the spheres and total proteins were extracted 48 h later, as previously described38 (link). After separation by SDS–polyacrylamide gel electrophoresis, proteins were blotted on nitrocellulose membranes (GE Healthcare Europe Gmb) and hybridized with the following antibodies: anti-HMGA1, anti-HMGA2 (Genetex) or affinity-purified anti-HMGA2, anti-α-actin (SantaCruz Biotechnologies).
Su L., Bryan N., Battista S., Freitas J., Garabedian A., D’Alessio F., Romano M., Falanga F., Fusco A., Kos L., Chambers J., Fernandez-Lima F., Chapagain P.P., Vasile S., Smith L, & Leng F. (2020). Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays. Scientific Reports, 10, 18850.
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3

Ectopic Gene Expression and Immunoblotting

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Ectopic genes were introduced to cells by retrovirus-mediated gene transfer (Young et al. 2009 (link)) using the retroviral vectors pLNCX2-Neo (ER:H-RASG12V) and pWZL-Hygro (E1A). Immunoblotting (Young et al. 2009 (link)) utilized the following antibodies: anti-H-RAS, anti-E1A and anti-HMGA2 (Santa Cruz); anti-ß-actin, anti-cyclin A2, and anti-p53 (Sigma); anti-PARP (Cell Signaling).
Madhu B., Narita M., Jauhiainen A., Menon S., Stubbs M., Tavaré S., Narita M, & Griffiths J.R. (2015). Metabolomic changes during cellular transformation monitored by metabolite–metabolite correlation analysis and correlated with gene expression. Metabolomics, 11(6), 1848-1863.
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