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Cd11c phycoerythrin

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CD11c-phycoerythrin is a fluorescent-labeled antibody that binds to the CD11c protein, which is expressed on the surface of dendritic cells and some other immune cells. It can be used for the identification and analysis of these cell populations in flow cytometry applications.

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Lab products found in correlation

Cd206 alexa fluor 647, by BioLegend (2 mentions) Fc block, by BD (2 mentions) Cd68 apc, by BioLegend (1 mentions) Cd206 fitc, by BioLegend (1 mentions) Cd45 apc cy7, by BioLegend (1 mentions) Cd11b pe, by BioLegend (1 mentions) Cellometer, by Revvity (1 mentions) Facscalibur, by BD (1 mentions) F4 80 alexa fluor 488, by BioLegend (1 mentions) Cd11b percp cy5, by BioLegend (1 mentions) F4 80 fitc, by BioLegend (1 mentions)

Close spelling variants of this product

CD11c-phycoerythrin (CD11b-PE,

3 protocols using cd11c phycoerythrin

1

Quantification of Immune Cell Subsets

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The numbers of SVCs in adipose tissue and blood samples in 5 mM EDTA were determined using a cellometer (Nexcelom Bioscience LLC). Samples were incubated for 10 min with BD Fc Block (BC Pharmingen) at a ratio of 1 : 100. Fluorophore-conjugated antibodies were added, and the mixtures were incubated in the dark for 20 min. A FACSCalibur (BD Biosciences) instrument was used to analyze the samples. The following antibodies were used for staining: CD45-APC Cy7 (BioLegend), CD68-APC (BioLegend), CD11c-phycoerythrin (BioLegend), CD206-FITC (BioLegend), CD45-APC Cy5.5 (BioLegend), and CD11b-PE (BioLegend). The percentage of cells stained with each was further analyzed using FlowJo (Tree Star).
Lee D., Yoo J.H, & Lee B.C. (2018). Therapeutic Effect of Cucumis melo L. Extract on Insulin Resistance and the Gut Microbiome in Lepob/Lepob Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 8159261.
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2

Multicolor Flow Cytometry Panel

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Fc Block (20 m g/mL; BD Biosciences) was used to block cell-surface antigens. After blocking cells were stained with fluorophore conjugated antibodies or isotype control antibodies. Fluorophore-conjugated primary antibodies were purchased from BioLegend: F4/80-Alexa Fluor 488, CD11b-PerCP/Cy5.5, CD11c-phycoerythrin, and CD206-Alexa Fluor 647. After incubation with antibodies, cells were washed and centrifuged, re-suspended in a washing buffer, and analyzed on a FACSCalibur using FlowJo 10.0.6.
Lin Y.W., Montassier E., Knights D, & Wei L.N. (2016). Gut microbiota from metabolic disease-resistant, macrophage-specific RIP140 knockdown mice improves metabolic phenotype and gastrointestinal integrity. Scientific Reports, 6, 38599.
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3

Isolation and Characterization of Wound Macrophages

Cited 1 time
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Macrophages in the wounds were isolated by enzymatic digestion [29 (link)]. Cell surface antigens were blocked with Fc block (20 ug/mL; BD Biosciences), incubated with fluorophore-conjugated antibodies or isotype control antibodies for 1h. Fluorophore-conjugated primary antibodies were from BioLegend: F4/80-FITC (#123122), CD11c-phycoerythrin (#117308) and CD206-Alexa Fluor 647 (#141712). Cells were washed and centrifuged at 500 g for 5 minutes and re-suspended in 1 ml washing buffer for FACS Calibur analyses using WinMDI software and gating strategy was described as previous [28 (link)]. Briefly, wound macrophages were collected and mononuclear cells were gated for FSC/SSC. Dead cell fractions were removed. The gated CD11b+F4/80+ cells were examined with anti-CD11c (as M1) or anti-CD206 (as M2) antibody.
Lin Y.W., Lee B., Liu P.S, & Wei L.N. (2015). Receptor-Interacting Protein 140 Orchestrates the Dynamics of Macrophage M1/M2 Polarization. Journal of Innate Immunity, 8(1), 97-107.
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