Elecsys probnp 2 reagent kit

All patients were taken medical history; meticulous physical examination; assessment of vital signs such as pulse and systolic and diastolic blood pressure; jugular venous distention, S3, murmurs, rales, and edema. It was then tested: first day serum creatinine (creatinine D1) and third day serum creatinine (creatinine D3) with Alinity c creatinine reagent running on Abbott's Alinity machine; plasma NGAL with human NGAL ELISA kit 036RUO of BioPorto Diagnostics A/S Copenhagen, Denmark; NT-proBNP with the Elecsys® proBNP II reagent kit from Roche Diagnostics, Bromma, Sweden, running on a Cobas e411 analyzer; these tests were performed at the laboratory department of Medic Medical Center 254 Hoa Hao Street, District 10, Ho Chi Minh City, Vietnam. Additional tests cell blood counts, urea, AST, ALT, electrolytes panel, and arterial blood gas were performed at the laboratory department of 115 People Hospital. Electrocardiography, chest X-ray, echocardiography, medications on admission and follow-up during hospital stay : length of hospital stay and in-hospital mortality, or serious illness were recorded. The estimated glomerular filtration rate was calculated using the 2009 CKD-EPI creatinine formula (eGFR_CKDEPI).

At the baseline, patient demographics, clinical status and treatment strategies were collected by review of electronic medical records. Heart function was assessed by World Health Organization functional classifications (WHO-FC). A 6 min walk distance (6MWD) was performed according to American Thoracic Society guidelines (18 (link)).
Biochemical markers were obtained, and the level of plasma N-terminal pro B-type natriuretic peptide (NT-proBNP) was measured by an enzyme-linked immunosorbent assay using a COBAS 6000 E601 immunoassay analyzer and an Elecsys proBNP II reagent kit (Roche Diagnostics GmbH, Mannheim, Germany).

The baseline laboratory data were acquired from the hospital's database. Blood samples for Ang‐2 without anticoagulant were collected in the first morning after admission and were centrifuged at 2600 g for 10 minutes. The serum supernatant was removed and stored at −80°C until it was used for analysis. Ang‐2 concentrations were measured with commercially available enzyme‐linked immunosorbent assay kits (RayBiotech, Inc). NT‐proBNP was measured with the Elecsys proBNP II reagent kit (Roche Diagnostics GmbH). High‐sensitive troponin‐I (hsTnI) was measured with the ARCHITECT STAT high‐sensitive troponin‐I assay (99th‐percentile‐cutoff: 26 ng/L). Estimated glomerular filtration rate (eGFR) was calculated using CKD‐EPI_(Scr‐CysC) Equation 16.