SCOC4, which indicated the best bioactivity, was analyzed for its monosaccharide composition by Carbohydrate Bioproduct Research Center, Sejong University, South Korea. Briefly, SCOC4 was hydrolyzed using trifluoroacetic acid at 105 °C, and the acid was evaporated under a vacuum. The sample was diluted (0.02%) and resolved using a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) system with a CarboPac ™ PA1 column intergraded to a Dionex ED50 Detector. Monosaccharides were identified and quantified by comparing with retention times of standard curves.
Ed50 detector
Manufactured by Thermo Fisher Scientific
The ED50 Detector is a laboratory instrument designed to measure and detect the presence of various analytes. It utilizes an electrochemical detection method to provide quantitative analysis of sample compositions. The core function of the ED50 Detector is to enable precise and reliable measurement of target compounds within a sample.
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Lab products found in correlation
2 protocols using ed50 detector
1
Monosaccharide Composition Analysis of SCOC4
2
HPLC Analysis of Biogenic Amines and Vitamins
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5-HIAA and HVA were separated and analysed by reverse phase HPLC using an octadecylsilyl column (250 × 4.6 mm, C-18) and with electrochemical detection (Dionex ED50 detector) by following protocol reported by Batllori et al (2017) [2] . 5-HIAA elutes before HVA in the order of increasing hydrophobicity (Heales, 2008) [6] . Thiamine, 5-MTHF (Blau and Opladen, 2008) [3] and PLP (Batllori et al., 2017) [2] were analysed by HPLC with electrochemical and fluorescence detection using ODS column (250 × 4.6 mm).
Standards were obtained from Sigma, St. Louis, MO, USA and stock standards were made to a final concentration of 500 µM. The coefficient of variation (CV= [Standard deviation/average] ×100%) from 15 samples was initially calculated to test the accuracy of methods and it resulted out to be less than 10% for both biogenic amines and vitamins. Thus, it was considered that the effect of blood contamination on CSF samples was insignificant when it was lower than 10% in comparison to the values in the non-spiked CSF samples.
Standards were obtained from Sigma, St. Louis, MO, USA and stock standards were made to a final concentration of 500 µM. The coefficient of variation (CV= [Standard deviation/average] ×100%) from 15 samples was initially calculated to test the accuracy of methods and it resulted out to be less than 10% for both biogenic amines and vitamins. Thus, it was considered that the effect of blood contamination on CSF samples was insignificant when it was lower than 10% in comparison to the values in the non-spiked CSF samples.
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