Catalase and superoxide dismutase activities were determined using the Catalase assay kit S0051 and the Superoxide Assay Kit S0060 from Beyotime (Beijing, China) according to the manufacturer’s instructions. Cell cultures with arabinose induced were harvested via centrifugation after 12 h of cultivation. After washing with 50 mM of PBS buffer (pH 7.0) 2 times, the cells were disrupted in an ultra-sonic crusher with the proper amount of β-mercaptoethanol added, and the crude extract was used to conduct the enzyme activity assay. The total protein amount was estimated using the Coomassie Brilliant Blue Staining method with a standard curve from the estimation of Bovine Serum Albumin.
Catalase assay kit s0051
Manufactured by Beyotime
Sourced in China
The Catalase Assay Kit S0051 is a laboratory equipment product designed to measure the activity of the enzyme catalase in biological samples. Catalase is an important antioxidant enzyme that plays a crucial role in the decomposition of hydrogen peroxide (H2O2) into water and oxygen. The kit provides the necessary reagents and protocols to quantify the catalase activity in a quick and reliable manner.
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Lab products found in correlation
2 protocols using catalase assay kit s0051
1
Antioxidant Enzyme Activity Assay
2
Oxidative Stress Modulation in MN9D Cells
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MN9D cells (3×105 cells/well) were treated with 1 mmol/L MPP+ and/or 5 μmol/L Cath-KP for an additional 24 h after being grown in 6-well plates for 24 h at 37 °C. The cells were then collected and split with RIPA lysis buffer (P0013, Beyotime Biotechnology, China). According to the manufacturer’s instructions, levels of SOD, CAT, and NO were detected using assay kits (Cu/Zn-SOD and Mn-SOD Assay Kit with WST-8 (S0103), Catalase Assay Kit (S0051), and Nitrate/Nitrite Assay Kit (S0023), respectively) from Beyotime Biotechnology (China).
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