Falcon 40 μm strainer

Manufactured by Corning

Sourced in United States

The Falcon 40-μm strainer is a cell strainer designed for the filtration of cell suspensions. It features a 40-micron nylon mesh filter that allows the passage of single cells while retaining larger cell aggregates, debris, and clumps. The strainer is made of high-quality, durable materials and is intended for use in various cell culture and cell preparation applications.

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Lab products found in correlation

Waters 2489 uv vis detector, by Waters Corporation (1 mentions) Waters alliance e2695 hplc system, by Waters Corporation (1 mentions) Cd11c microbeads ultrapure, by Miltenyi Biotec (1 mentions) Macs column, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

40-μm strainer (13 citations) Falcon® cell strainers (40 μM) (3 citations)

2 protocols using falcon 40 μm strainer

Characterization of PM2.5 Composition

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PM₂.₅ was collected by a Laoying 2030 air sampler (Laoshan Institute of Applied Technology, Qingdao, China) in Taizhou. The collection was adhered to Teflon-coated quartz fiber filters and then cut into pieces. The pieces were washed with PBS three times on ice and filtered again by a Falcon 40-μm strainer (Corning, NY, United States). The filtrate was freeze-dried in a vacuum and resuspended in PBS at a concentration of 5 mg/ml. PM₂.₅ was finally stored at −20°C.
By analysis with a Waters Alliance e2695 HPLC system connected to a Waters 2489 UV/Vis Detector (MA, United States) in a laboratory at Taizhou Environmental Monitoring Center (Jiangsu, China), polycyclic aromatic hydrocarbon (PAH) complexes were determined to be the main component of PM₂.₅. The analysis followed a standard protocol for the determination of particulate phase PAHs (HJ647-2013, Ministry of Environmental Protection, China) (Chen et al., 2019 (link)).

Sun Z., Ji N., Jiang J., Tao Y., Zhang E., Yang X., Wang Z., Chen Z., Huang M, & Zhang M. (2021). Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection. Frontiers in Microbiology, 11, 525976.

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Enriched Dendritic Cell Isolation

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To improve the yield of DCs, C57BL/6 female mice were injected s.c. with 7 X 10⁵ B16.Flt3L, a cell line genetically engineered to stably secrete fms-related tyrosine kinase 3 ligand (Flt3L) (27 (link),33 (link)). Spleens were harvested 14 days later and processed as described (34 (link)). Briefly, to prepare single cell suspensions, spleens were minced in RPMI, and the obtained mixture was filtered using a Falcon 40 μM strainer (Corning). Following red blood cell lysis by ACK buffer, the cell suspension was filtered again. After washing in PBS, cells were blocked with CD16/32 antibody to Fc gamma III and II receptors (Thermo Fisher Scientific; Clone 93), followed by. incubation with CD11c microbeads ultrapure (Milteny Biotec) and loaded onto a MACS column (Milteny Biotec) for the positive selection, as outlined in the kit protocol.

Diamond J.M., Vanpouille-Box C., Spada S., Rudqvist N.P., Chapman J.R., Ueberheide B.M., Pilones K.A., Sarfraz Y., Formenti S.C, & Demaria S. (2018). Exosomes shuttle TREX1-sensitive IFN-stimulatory dsDNA from irradiated cancer cells to DCs. Cancer immunology research, 6(8), 910-920.

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