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Mouse tslp elisa max deluxe set

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The Mouse TSLP ELISA MAX Deluxe™ Set is a laboratory product designed for the quantitative measurement of mouse thymic stromal lymphopoietin (TSLP) in biological samples. It provides the necessary components to perform an enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Luminex 200 system, by Merck Group (1 mentions) 17 plex mouse procartaplex panel, by Thermo Fisher Scientific (1 mentions) Tissuelyser 2, by Qiagen (1 mentions)

2 protocols using mouse tslp elisa max deluxe set

1

Multiplex Cytokine Analysis in Mouse Ears

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Ears (1 per mouse) were mechanically disrupted on a TissueLyser II (Qiagen) in 0.75 ml T-PER protein extraction reagent (Pierce) and soluble proteins were quantified by BCA protein assay (Pierce). Cytokines were measured using the Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (ThermoFisher) according to manufacturer’s instructions and data was acquired using a Luminex 200 system (Millipore). TSLP, IL-4, and IL-5 were assessed by ELISA according to manufacturers’ instructions using Mouse TSLP ELISA MAX Deluxe™ Set (BioLegend), Mouse IL-4 ELISA Ready-SET-Go! (eBioscience), and BD OptEIA™ Set Mouse IL-5 (BD Biosciences), respectively.
Shane H.L., Lukomska E, & Anderson S.E. (2019). Topical application of the quaternary ammonium compound didecyldimethylammonium chloride activates type 2 innate lymphoid cells and initiates a mixed-type allergic response. Toxicological sciences : an official journal of the Society of Toxicology, 168(2), 508-518.
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2

Quantification of Cytokines in Mouse Serum and Cell Culture

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The contents of IL-17, IL-23 and TSLP in mouse serum were quantified by commercially available ELISA kits according to the manufacturers' instructions. The following ELISA kits were used: mouse IL-17A/F ELISA MAX™ Deluxe Set (BioLegend, cat: 436204); mouse IL-23 ELISA MAX™ Deluxe Set (BioLegend, cat: 433704) and mouse TSLP ELISA MAX™ Deluxe Set (BioLegend, cat: 434104). 20 μL of serum was diluted with 80μL of 1X Assay Diluent A and then was added to one well of a 96-well plate for the assay. 100 μL of culture media of HaCaT cells was added to one well of a 96-well plate for measurement of TSLP or TGF-β1 level using either human TSLP ELISA MAX™ Deluxe Set (BioLegend, cat: 434204) or TGF-β1 ELISA MAX™ Deluxe Set (Elabscience, cat: E-EL-0162c).
Zheng J., Wang H., Wang J., Peng Z., Yao X., Weber H.C., Qin X., Xiang Y., Liu C., Ji M., Liu H, & Qu X. (2024). Bombesin receptor-activated protein homolog deficiency altered the pattern of pathological changes of psoriasis - like skin lesion in mice. International Journal of Medical Sciences, 21(2), 357-368.
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