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Thiobarbituric acid tba

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Thiobarbituric acid (TBA) is a chemical reagent commonly used in biochemical analyses. It is a colorimetric assay used to detect and quantify the presence of malondialdehyde, a byproduct of lipid peroxidation. This assay is often used to measure oxidative stress in various biological samples.

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Lab products found in correlation

Potassium chloride (kcl), by Merck Group (2 mentions) Trichloroacetic acid tca, by Merck Group (2 mentions) Maxima sybr green rox qpcr master mix 2x, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) H3po4, by Merck Group (1 mentions) N butanol, by Merck Group (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Genejet rna purification kit, by Thermo Fisher Scientific (1 mentions) Sucrose, by Serva Electrophoresis (1 mentions) Mgcl2, by Serva Electrophoresis (1 mentions) Na2seo3, by Merck Group (1 mentions) 2 2 diphenyl 1 picryl hydrazyl hydrate dpph, by Merck Group (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Phosphoric acid, by Merck Group (1 mentions) Alcl3, by Merck Group (1 mentions) Ammonium molybdate, by Merck Group (1 mentions) Millipore water purification system, by Merck Group (1 mentions) Simvastatin, by Merck Group (1 mentions)

5 protocols using thiobarbituric acid tba

1

Oxidative Stress Biomarker Quantification

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All the standards, reagents, and solvents used throughout the experiments were of analytical grade. Na2SeO3, KCl, Tris, HCl, H3PO4, n-butanol, and 2-mercaptoethanol were from Sigma-Aldrich (Steinheim, Germany); TBA (thiobarbituric acid), sucrose, MgCl2, HNO3, and H2O2 were purchased from Serva (Heidelberg, Germany); Maxima SYBR Green/ROX qPCR Master Mix (2X), “Gene JET RNA purification kit”, and “High Capacity cDNA Reverse Transcription kit” were from Thermo Fisher Scientific (Waltham, MA, USA).
Staneviciene I., Sulinskiene J., Sadauskiene I., Liekis A., Ruzgaite A., Naginiene R., Baranauskiene D., Simakauskiene V., Krusnauskas R, & Viezeliene D. (2022). Effect of Selenium on the Iron Homeostasis and Oxidative Damage in Brain and Liver of Mice. Antioxidants, 11(7), 1216.
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2

Analytical Methods for Cannabinoid Determination

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Potassium chloride, phosphoric acid, and hydrogen peroxide were purchased from Merck (Darmstadt, Germany). Ethanol (96%) was supplied from Vilnius Degtine (Vilnius, Lithuania). Purified water was produced using a Millipore water purification system (Merck, Kenilworth, NJ, USA). Ammonium molybdate, ammonium formate ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate), and DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) reagents were purchased from Sigma-Aldrich (Schnelldorf, Germany). Acetonitrile was purchased from Sigma-Aldrich (Buchs, Switzerland). Formic acid was purchased from Fluka Chemie (Buchs, Switzerland). DTNB (5,5-dithiobis-(2-nitrobenzoic acid), tris-HCl (tris-hydrochloride), and TBA (thiobarbituric acid) were purchased from Serva (Heidelberg, Germany). Standard solutions of cannabinoids (CBD, CBDA, CBG) and AlCl3 (>99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Kubiliene A., Mickute K., Baranauskaite J., Marksa M., Liekis A, & Sadauskiene I. (2021). The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo. Life, 11(7), 647.
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3

Evaluating Statin Effects on Cellular Metabolism

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4-(2-hydroxyethyl) 1-piperazine-ethanessul-fonic acid (HEPES) was obtained from Acros (New Jersey, USA). Albumin bovine type was purchased from Roche diagnostic corporation (Indianapolis, USA). Statins (lovastatin, simvastatin and atorvastatin), 2,7-dichlorofluorescein diacetate, rhodamine 123 and collagenase from clostridium histolyticum were obtained from Sigma Aldrich (St. Louis, USA). Trichloro acetic acid (TCA), Ethyleneglycol-bis (ρ-aminoethylether)-N,N,N′,N′-tetra acetic acid (EGTA) and Trypan blue were obtained from Merck (Darmstadt, Germany). Thiobarbituric acid (TBA) was obtained from SERVA (Heidenberg, New York, USA). All salts used for preparing buffer solutions were of analytical grade and obtained from Merck (Darmstadt, Germany).
Abdoli N., Azarmi Y, & Eghbal M.A. (2015). Mitigation of statins-induced cytotoxicity and mitochondrial dysfunction by L-carnitine in freshly-isolated rat hepatocytes. Research in Pharmaceutical Sciences, 10(2), 143-151.
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4

Evaluating Liver Injury Biomarkers

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N-acetyl cysteine (NAC), and Dithioteritol were obtained from Acros (New Jersey, USA). Trichloroacetic acid (TCA), Ethylenediaminetetraacetic acid (EDTA), Vitamin E, and Vitamin C were obtained from Merck (Darmstadt, Germany). Kits for evaluating biomarkers of liver injury were obtained from Pars Azmun (Tehran, Iran). Thiobarbituric acid (TBA) was obtained from SERVA (Heidenberg, New York). All salts used for preparing buffer solutions were of analytical grade and obtained from Merck (Darmstadt, Germany).
Heidari R., Esmailie N., Azarpira N., Najibi A, & Niknahad H. (2016). Effect of Thiol-reducing Agents and Antioxidants on Sulfasalazine-induced Hepatic Injury in Normotermic Recirculating Isolated Perfused Rat Liver. Toxicological Research, 32(2), 133-140.
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5

Antioxidant Assay Protocols

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Bodipy™ 581/591 C11 was obtained from ThermoFisher. 4',5,7-Trihydroxy-6-methoxyflavone (hispidulin) was purchased from Medchemexpress. 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2'-azino-bis(3-ethylobenzthiazoline-6sulfonic acid, diammonium salt) (ABTS), 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), potassium persulfate, sodium ethylenediaminetetraacetate (EDTA) and ferric chloride were from Merck (Poznań, Poland). Phosphate-buffered saline (PBS), sodium dodecyl sulfate (SDS), monosodium phosphate, disodium phosphate and dimethylsulfoxide (DMSO) were from Bioshop (Burlington, ON, Canada).
Thiobarbituric acid (TBA) was from Serva Electrophoresis GmbH (Heidelberg, Germany). Methanol, acetic acid, hydrochloric acid and trichloroacetic acid were from Chempur (Piekary Śląskie, Poland).
Sodium acetate was from POCh (Gliwice, Poland).
Transparent and black flat-bottom 96-well plates (Greiner, Kremsmünster, Austria) were used for the assays. Absorbance and fluorescence was measured in a Spark plate reader (BioTek, Winooski, VT, USA).
Kut K., Bartosz G., Soszyński M, & Sadowska-Bartosz I. (2022). Antioxidant properties of hispidulin. Natural product research, 36(24).
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