Fourier 300 mhz ft nmr spectrometer

The 7-hydroxyflavone and 5,7-dihydroxyflavone were purchased from INDOFINE Chemical Company, Inc. (Hillsborough, NJ), and other chemicals were purchased from Sigma-Aldrich Corporation (St. Louis, MO) and Fisher Scientific International, Inc. (Hampton, NH). All the thermal cyclization reactions were performed with a CEM Discover microwave synthesis system (Matthews, NC). Mass spectral data were determined using an Agilent 6890 GC instrument with a 5973 MS instrument. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Fourier 300 MHz FT-NMR spectrometer. Elemental analyses were performed by Atlantic Microlab, Inc. (Norcross, GA). On the basis of elemental analyses, the purity of all tested compounds is higher than 95%.

All reagents, solvents, and analytical standards were purchased from Sigma-Aldrich (St. Louis, MO), Fisher Scientific (Fairfield, NJ), AK Scientific (Union City, CA), and CombiPhos Catalysts (Princeton, NJ) and were used as received. ¹H NMR spectra were recorded on a Bruker Fourier 300 MHz FT-NMR spectrometer. ¹³C NMR spectra were obtained on an Agilent 400-MR NMR spectrometer, and the data was processed using MestReNova NMR software (School of Chemistry, University of Bristol, Bristol, UK). Chemical shifts are reported as parts per million (ppm) relative to TMS. Specific rotation was determined with an AUTOPOL III polarimeter (Rudolph Research Analytical, NY). HRMS spectra data were collected on a Thermo LTQ Orbitrap-XL mass spectrometer in positive ion mode. The tested compound was confirmed to be >95% pure by HPLC.