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Las 1000 plus gel documentation system

Manufactured by Fujifilm
Sourced in Japan

The LAS-1000 Plus Gel Documentation System is a laboratory equipment designed for the analysis and documentation of DNA, RNA, and protein gels. It provides high-resolution imaging and analysis capabilities for various gel-based applications.

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3 protocols using las 1000 plus gel documentation system

1

Mycelia DNA Blot Analysis of C. orbiculare

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The total DNA from the mycelia of C. orbiculare was isolated, and a DNA blot analysis was performed using a previously described method [22] (link). DNA digestion, gel electrophoresis, labeling of probes, and hybridization were performed according to the manufacturer’s instructions following standard methods [20] . DNA probes were labeled with DIG-dUTP using a BcaBESTTM DIG labeling kit (Takara Bio, Ohtsu, Japan). Hybridized DNA was detected with anti-Digoxygenin antibody Fab fragments conjugated to alkaline phosphatase (Roche Diagnostics, Tokyo, Japan). Light emission from the enzymatic dephosphorylation of the CDP-Star Detection Reagent (GE Healthcare, Tokyo, Japan) was detected using the Fujifilm LAS-1000 Plus Gel Documentation System (Fujifilm, Tokyo).
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2

Genomic DNA Analysis of C. orbiculare

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Genomic DNA of C. orbiculare was isolated from mycelia, and DNA blot analysis was done by standard methods. DNA probes were labeled with DIG-dUTP using the BcaBEST DIG Labeling Kit (Takara Bio). Hybridized DNA was detected using Anti-Digoxygenin-AP Fab fragments (Roche Diagnostics), and light emission generated by enzymatic dephosphorylation of CDP-Star Detection Reagent (GE Healthcare) by alkaline phosphatase was detected using the FUJIFILM LAS1000 plus gel documentation system (Fujifilm).
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3

Cell Lysis and Western Blot Analysis

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Cells were washed with cold phosphate buffered saline (PBS) and lysed with a lysis buffer [50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 × protease inhibitor cocktails (Roche Applied Science, Basal, Switzerland), 1 × phosphatase inhibitor mixture (Calbiochem), and 1 % NP-40] on ice for 10 min. Cell lysates were clarified by centrifugation at 10,000 × g for 10 min at 4 °C, and the supernatants were collected for western blotting. Protein lysate concentration was determined using the DC protein assay kit (Bio-Rad, Hercules, CA). The supernatants were separated by SDS-PAGE. Western blotting was performed using the ECL-Plus immunoblotting detection system (GE Healthcare UK Ltd., Amersham Place, England) in accordance with the manufacturer’s instructions. The relative density of each immunoreactive band was quantified using LAS-1000 plus gel documentation system (Fuji Film Co., Fuji, Japan).
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