Human angiogenesis growth factor magnetic bead panel 1

After an overnight fast (at least 12 h), blood samples were drawn from the antecubital vein. Blood samples in tubes containing EDTA were spun immediately at 1000g for 10 min. Plasma was isolated and stored at -80°C until analyses in the Center of Biomedical Research (Granada, Spain). 22 The analysis of mature BDNF (μg/L), IGF-1 (ng/mL), VEGFA (pg/L), and EGF (pg/L) in plasma was performed using the Luminex IS 100/200 system (Luminex Corporation, Austin, TX), with the XMap technology and using human monoclonal antibodies (Milliplex Map Kit, Millipore, Billerica, MA). BDNF concentration was measured (Human Neurodegenerative Disease Magnetic Bead Panel 3; EMD Millipore Corporation, Billerica, U.S.A.) with a sensitivity of 0.23 pg/mL, and intra-and inter-assay precision coefficients of variation (CVs) of <5.4% and <5.3%, respectively. The IGF-1 concentration was analysed (Human IGF-I, II Magnetic Bead Panel, Millipore Corporation, Billerica, U.S.A.) with a sensitivity of 15 pg/mL and intra-and inter-assay CVs of <10% and <15%, respectively. VEGFA and EGF were quantified (Human Angiogenesis / Growth Factor Magnetic Bead Panel 1, EMD Millipore Corporation, Billerica, U.S.A.) with a sensitivity of 8.1 pg/mL for VEGFA, and 1.0 pg/mL for EGF. The intra-and inter-assay precision CVs were 3.5% and 10% for VEGFA, and 3.2% and 6.8% for EGF, respectively.