The following antibodies were used for western blot analysis; anti-GDF15 antibody (cat.no. ab223539; Abcam), anti-snail antibody (cat.no. ab53519; Abcam), anti-slug antibody (cat.no. ab106077; Abcam), anti-β-actin antibody (cat.no. ab8224; Abcam), anti-E-cadherin antibody (cat.no. ab40772; Abcam), anti-Vimentin antibody (cat.no. ab92547; Abcam), anti-SCAP antibody (cat.no. ab125186; Abcam), anti-SREBF2 antibody (cat.no. ab30682; Abcam), and anti-HMGCR antibody (cat.no. ab174830; Abcam). Antibodies used for immunohistochemistry analysis were as follows; anti-GDF15 antibody (cat.no. ab223539; Abcam), anti-E-cadherin antibody (cat.no. ab40772; Abcam), anti-Vimentin antibody (cat.no. ab92547; Abcam), anti-SCAP antibody (cat.no. ab125186; Abcam), anti-SREBF2 antibody (cat.no. ab30682; Abcam), and anti-HMGCR antibody (cat.no. ab174830; Abcam). β-cyclodextrin (β-CD) and Cholesterol-Water Soluble powder was purchased from Sigma-Aldrich (St. Louis, MO). Recombinant human GDF15 (rhGDF15) was purchased from R&D Systems (Minneapolis, MN). Matrigel was purchased from Advanced BioMatrix (San Diego, CA). High-cholesterol diet was purchased from Trophic Animal Feed High-Tech Co., Ltd. (Nantong, China).
Dong G., Huang X., Jiang S., Ni L., Ma L., Zhu C, & Chen S. (2020). SCAP Mediated GDF15-Induced Invasion and EMT of Esophageal Cancer. Frontiers in Oncology, 10, 564785.
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