Fabs were expressed and purified as reported previously (57 (link)). Briefly, genes encoding antibody clones were cloned into a vector that expresses a Fab with the Avi-tag at the C-terminus of the heavy chain. Fabs were produced in E. coli strain 55244 (ATCC) and purified using a HiTrap Protein G affinity column (Cytiva). Purity >90% was confirmed using SDS-PAGE.
Hitrap protein g affinity column
Manufactured by Cytiva
The HiTrap Protein G affinity column is a pre-packed, ready-to-use column designed for the purification of antibodies and other Fc-containing proteins. The column contains Protein G immobilized on agarose beads, which selectively binds to the Fc region of immunoglobulins. This allows for the rapid and efficient capture and purification of antibodies from complex samples.
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Lab products found in correlation
2 protocols using hitrap protein g affinity column
1
Fab Expression and Purification in E. coli
2
Fab Expression and Purification in E. coli
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