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Pkh67 green fluorescence labelling kit

Manufactured by Merck Group
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Sourced in United States

The PKH67 green fluorescence labelling kit is a laboratory product designed for cell labelling. It utilizes a fluorescent dye that can be incorporated into the lipid regions of the cell membrane, allowing for the visualization and tracking of cells using fluorescence microscopy or flow cytometry. The kit provides the necessary reagents and protocol for this labelling process.

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Lab products found in correlation

Microcon ym 100, by Merck Group (1 mentions)

Spelling variants of this product

PKH67 (477 citations) PKH67 kit (16 citations) PKH67 green (14 citations) PKH67 green fluorescence kit (2 citations) PKH67 green fluorescence (2 citations)

2 protocols using pkh67 green fluorescence labelling kit

1

Labeling and Intratracheal Delivery of HBEC EVs

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Purified HBEC EVs were labelled with a PKH67 green fluorescence labelling kit (Sigma‐Aldrich). HBEC EVs (2.0×109) were incubated with 2 mM PKH67 for 5 min and then washed five times using a 100 kDa filter (Sartorius, VIVACON 500 100K VN01H41) to remove excess dye. PKH67‐labelled EVs were intratracheally administered in 50 μl saline on day 7, and the lungs were collected on the next day.
Kadota T., Fujita Y., Araya J., Watanabe N., Fujimoto S., Kawamoto H., Minagawa S., Hara H., Ohtsuka T., Yamamoto Y., Kuwano K, & Ochiya T. (2021). Human bronchial epithelial cell‐derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF‐β‐WNT crosstalk. Journal of Extracellular Vesicles, 10(10), e12124.
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2

Fluorescent Labeling of Extracellular Vesicles

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Purified EVs derived from ES-2, RMG-1 and HOSE1 cells were labelled with a PKH67 green fluorescence labelling kit (Sigma-Aldrich, MO, USA). EVs were incubated with 2 μM of PKH67 for 5 min and washed five times using a 100 kDa filter (Microcon YM-100, Millipore) to remove excess dye. PKH67-labelled EVs were used to assess EV uptake in vitro and in vivo.
Yokoi A., Yoshioka Y., Yamamoto Y., Ishikawa M., Ikeda S.I., Kato T., Kiyono T., Takeshita F., Kajiyama H., Kikkawa F, & Ochiya T. (2017). Malignant extracellular vesicles carrying MMP1 mRNA facilitate peritoneal dissemination in ovarian cancer. Nature Communications, 8, 14470.
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