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7 protocols using n amyl alcohol

1

Immobilized Candida antarctica Lipase B

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Lipase from Candida antarctica type B, produced by the fermentation of genetically modified microorganisms, was a generous gift from Genofocus (Daejeon, Republic of Korea). The commercial preparation CalB-IMTM (Genofocus, Daejeon, Republic of Korea) contains lipase from Candida antarctica B, adsorbed onto a microporous ion-exchange resin. The silane precursors used for the sol-gel entrapment of the native lipase were tetramethoxysilane (TMOS) from Acros Organics (Geel, Belgium), while the silanes with epoxy functional groups, (3-glycidoxypropyl)trimethoxysilane (GPTMS) 99+% (product code SIG5840.1), (3-glycidoxypropyl)bis(trimethylsiloxy)methylsilane (GP(TMS)2MS) 97% (product code SIG5820.0) and 1,3-bis(glycidoxypropyl)tetramethyldisiloxane ((GP)2TMDSO) 97% (product code SIB1115.0) were purchased from Gelest (Morrisville, PA, USA). The other materials used were tris-(hydroxymethyl)-aminoethane, 2-propanol, 1-octyl-3-methyl-imidazolium tetrafluoroborate (OmimBF4), n-amyl alcohol, caproic acid, n-hexane, Coomassie Brilliant Blue G-250, bovine serum albumin (BSA), sodium fluoride, potassium fluoride, n-dodecane > 99%, fluorescein isothiocyanate (FITC), acetone, and 2-methyltetrahydrofuran (2-MeTHF), which were purchased from Merck.
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2

Synthesis of Functionalized Silica Nanoparticles

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Tetraethyl orthosilicate (TEOS, ≥ 99%), cetyltrimethylammonium bromide (CTAB, ≥ 99%), (3-aminopropyl)triethoxysilane (APTES, 99%), bis[3-(triethoxysilyl)propyl] tetrasulfide (TESPTS, ≥ 90%), (3-mercaptopropyl)triethoxysilane (MPTES, ≥ 80%), acid Fuchsin (AF, dye content 70%), acid orange II (AO, ≥ 98%) were purchased from Sigma-Aldrich (Germany). Cyclohexane (≥ 99.9%), hydrochloric acid (HCl, 37%), urea (≥ 99%), n-amyl alcohol (≥ 98.5), and sodium hydroxide (NaOH, pellets, ≥ 97%) were purchased from Merck Millipore (Germany). Ethanol (96% and absolute) and acetone (HPLC grade) were purchased from Mojallali Chemical Co. (Tehran, Iran).
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3

Phytochemical Analysis of Plant Extracts

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Reagents used to analyze were analytical grade, including sodium acetate, chloroform, sulphuric acid, mercury chloride, potassium iodide, sodium hydroxide, folin ciocalteus phenol, cuppric sulphate, sodium nitrite, sodium dihydrogen phosphate, disodium hydrogen phosphate, methanol, eter, ethanol, ammonia solution, potassium ferric cyanide, trichloro acetic acid, acetic acid glacial, iodine, hydrochloride acid, n-amyl alcohol, magnesium powder, and ferric chloride were purchased by Merck Company (Darmstadt, Germany). Potassium sodium tartrate tetrahydrate, gallic acid, sodium carbonate, (+)-catechin, aluminium chloride, 2,2-diphenyl-1-picrylhydrazyl, alpha amylase enzyme, alpha glycosidase enzyme, amylum, and p-nitrophenyl-α-Dglucopyranoside were purchased from Sigma-Aldrich Company Ltd. (Gillingham, UK). Distillated water was supplied by PT Aqua Surabaya, Indonesia.
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4

Beetroot-Based Cosmetic Formulation

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The materials used in this study included: fresh beetroot (Beta vulgaris L.) obtained from Pasir Mulya organic Indonesia farm, talcum (90% purity, Pingdu Talc Mine, Shandong), oleum ricini (87.4% purity, Fagron, Rotterdam), acidum ascorbicum (99.8% purity, CSPC Weisheng Pharmaceutical), magnesium stearate (Faci Asia Pacific, Singapore), methylparaben (100.3%), vaseline album, oleum cacao (Bumi Tangerang Mesindotama, Tangerang), adeps lanae (Fagron, Rotterdam), 99.9% propylene glycol, cera alba (Caesar Loretz, Germany), cetyl alcohol (Ecogreen Oleochemicals, Jakarta), iron(III) chloride solution (Merck, Germany), formaldehyde 30% (Merck, Germany), HCl (Merck, Germany), NaOH (Merck, Germany), Mg 2 SO 4 (Merck, Germany), n-amyl alcohol (Merck, Germany), acetic acid (Merck, Germany), sulfuric acid (Merck, Germany), chloroform (Merck, Germany), sodium acetate (Merck, Germany), Dragendorff reagent, Mayer reagent and distilled water (Brataco).
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5

Preparation of High-Purity Organic Solvents

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High purity water was obtained by distillation using an Aquatron Automatic water still A4000 (Bibby Scientific, Staffordshire, UK) while analytical grade ethanol (99.9% v/v) and acetic acid (99.0% v/v) used as a working reference standards were from Scharlau (Sentmenat, Spain). Methanol (99.9% v/v) and n-amyl alcohol (99.9% v/v) were from Sigma-Aldrich (Steinheim, Germany) while acetaldehyde (99.8% v/v), ethyl acetate (98.0% v/v), isobutanol (99.0% v/v) and isoamyl alcohol (99.0% v/v) were from Merck (Darmstadt, Germany).
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6

Diazinon Pesticide Quantification Protocol

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Analytical diazinon pesticide with a purity of 98.5%, Acetic acid 99.9%, ethanol 99.9%, chloride iron (II), chloride iron (III), tetra ethyl ortho silicate 95%, tetra-n-butyl lorthotitanate, ammonium solution, alamar blue powder, agar muller hinton, broth nutrient, dimethyl sulfur oxide (DMSO), n-amyl alcohol, HCl-phthalate buffer, glucose, sodium acetate, sodium bicarbonate, Sulfuric acid 98%, Sodium Hydroxide 98%, potassium phosphate monobasic and Dipotassium phosphate were purchased from Sigma Aldrich Co. The properties of diazinon and alamar blue are shown in Table 1.

Properties of diazinon and alamar blue

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7

Analytical Acetamiprid Toxin Characterization

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Analytical acetamiprid toxin was purchased from Sigma-Aldrich Co. with a purity of 98.5%, while its technical form was purchased from Ariashimi Co, Zahedan with a purity of 97%. Acetic acid, ethanol 99.9%, FeCl3, FeCl2, tetra ethyl ortho silicate 95%, tetra-n-butyl lorthotitanate, ammonium solution, Resazurin powder, agar muller hinton, broth nutrient, KH2PO4, K2HPO4, glucose, sodium acetate, dimethyl sulfur oxide (DMSO), n-amyl alcohol, HCl–phthalate buffer, and sodium bicarbonate were purchased from Sigma-Aldrich Co.
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