Rat myosin light chain 3 elisa kit

Myosin light chain 1 (MLC1) concentration in coronary effluents was determined with the use of Rat Myosin Light Chain 3 ELISA Kit (sensitivity: 6.9 × 10⁻³ ng/mL; Bioassay Technology Laboratory, Birmingham, United Kingdom). Briefly, capture antibody bound MLC1 from coronary effluents. Next, antigens were detected by biotinylated antibody. HRP conjugated with biotinylated MLC1 antibody. TMB substrate solution was used to develop the reaction. MLC1 concentration in coronary effluents was expressed as ng/L and normalized to CF.

ALC1 and VLC1 content in heart homogenates and coronary effluents were determined using a Rat Myosin Light Chain 4 (MYL4) ELISA Kit (sensitivity: 0.1 ng/mL; MyBioSource, San Diego, California, United States of America) and Rat Myosin Light Chain 3 ELISA Kit (sensitivity: 6.99 × 10⁻³ ng/mL; Bioassay Technology Laboratory, Shanghai, China). Briefly, capture antibodies bound ALC1/VLC1 from heart homogenates/coronary effluents. Next, antigens were detected by biotinylated antibodies. HRP conjugated with biotinylated ALC1/VLC1 antibody. TMB substrate solution was added to enable complex visualization. Concentrations of ALC1/VLC1 in heart homogenates were expressed as ng per µg of total protein and as ng/mL normalized to coronary flow in coronary effluents.

The concentration of ventricular isoform of myosin light chain 1 (MLC1) in perfusates was determined using Rat Myosin Light Chain 3 ELISA Kit (Bioassay Technology Laboratory, Shanghai, China). Briefly, primary capture antibodies bind MLC1 from the sample. Then, MLC1 is detected with biotinylated anti-MLC1 secondary antibodies and streptavidin-horseradish peroxidase (HRP) complex. The substrate solution is then added, and color develops in proportion to the amount of rat MLC1.
Cardiac troponin I (TnI) in coronary effluents was quantitatively measured using Rat Cardiac Troponin I SimpleStep ELISA Kit (Abcam, Cambridge, UK). Briefly, TnI is tied with antibody specific to rat cardiac muscle TnI and is detected by biotin-conjugated polyclonal antibody and HRP. Next, TMB Development Solution is used to enable visualization of the reaction. The concentration of MLC1 and TnI in coronary effluents was normalized to CF.