The sample was homogenized, resuspended to 0.25 g/L with 0.85% sodium chloride saline, and immobilized on polysaccharide gel beads for long-term pre-incubation15 (link). The stool gel beads were inoculated in an anaerobic culture bottle (BioMérieux, Marcy l’Etoile, France) supplemented with 0.2 μm filtered rumen fluid16 (link) and defibrinated sheep blood and then incubated at 37 °C for 30 days. The cultured broth was sampled regularly and plated onto modified Gifu Anaerobic Medium (mGAM; Nissui Pharmaceutical, Tokyo, Japan). Bacterial colonies were isolated and initially identified using MALDI-TOF MS on a Biotyper Sirius system (Bruker Daltonics, Bremen, Germany)17 (link). The process was conducted in an anaerobic chamber consisting of 5% CO2, 10% H2, and 85% N2.
Biotyper sirius system
Manufactured by Bruker
Sourced in Germany
The Biotyper Sirius system is a compact and automated microbial identification platform developed by Bruker. The core function of this system is to provide rapid and reliable identification of a wide range of microorganisms, including bacteria and yeasts, based on mass spectrometry technology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biotyper sirius system
1
Isolation and Identification of Anaerobic Gut Bacteria
2
A. baumannii Identification Workflow
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A. baumannii strains isolated from the patients included were recovered on Luria-Bertani agar and incubated overnight at 37 . The isolates were identified by a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Biotyper ® Sirius System (Bruker Daltonics GmbH, Bremen, Germany), and confirmed via whole genome-sequencing using an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA). Escherichia coli ATCC25922 and A. baumannii ATCC19606 were used as control strains.
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