Whatman n 1

Manufactured by Cytiva

Sourced in Germany

Whatman N° 1 is a qualitative filter paper commonly used in laboratory settings. It is designed to retain fine particulates while allowing liquids to pass through. The paper has a consistent, uniform structure and is suitable for a variety of filtration applications.

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Whatman, by Cytiva (5 mentions)

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Whatman (94557 citations) Whatman filter paper N°1 (25 citations) Whatman paper N°1 (20 citations) Whatman filter N°1 (4 citations) Whatman filters n.1 (4 citations)

5 protocols using whatman n 1

Quantification of Fusarium verticillioides

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Ten g of each ground feed samples were mixed with 90 mL sterile PBS and serial dilutions were carried out until 10⁻⁴ dilution. One mL of each dilution was added to Petri plates containing 25 mL PDA with chloramphenicol and tartaric acid and incubated at 28 °C for seven days. Subsequently, the genera were identified according to Nelson et al. [45 ] and Fusarium sp. was counted.
The remaining feed suspension was filtered (Whatman N° 1,Whatman GmbH, Dassel, NI, Germany) and stored at −20 °C for further quantification of F. verticillioides exoantigen by ic-ELISA.

Omori A.M., Ono E.Y., Hirozawa M.T., de Souza Suguiura I.M., Hirooka E.Y., Pelegrinelli Fungaro M.H, & Ono M.A. (2019). Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples. Toxins, 11(1), 48.

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Evaluation of Essential Oil Toxicity Against Insects

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The test was carried out according to the method described by [37 (link)]. Several preliminary tests were carried out to select the doses to be used for CAEOs. Four doses were prepared by diluting 1, 2, 3, and 4 µL of essential oils in 1 mL acetone, corresponding to doses of 0.016, 0.031, 0.050, and 0.063 µL/cm². One mL of each solution was dispensed on a 9 cm diameter (63.62 cm² surface area) filter paper disk (Whatman n°1) placed in a glass Petri dish of the same diameter. For the control, filter papers were treated with acetone only. After 10 min, once the solvent had been evaporated, 10 unsexed adults freshly collected from their rearing environment, aged 7 to 14 days, were introduced into each Petri dish, which were then resealed. Three replicates were performed for e each dose. Mortality was recorded after 24 h. Insects were considered dead if no movement of legs or antennae was recorded.

Kasrati A., Sakar E.H., Aljaiyash A., Hirri A., Tamegart L., Abbad I, & Alaoui Jamali C. (2024). Chemical Profiling, Insecticidal, and Phytotoxic Effect of Essential Oils from Leaves and Inflorescence of Moroccan Chenopodium ambrosioides (L.). Plants, 13(4), 483.

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Deoxynivalenol Quantification in Wheat

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At harvest, wheat heads were collected to determine DON concentration in the entire heads. Toxin extraction was done using Mycosep 225 (Romer Labs) according to the manufacturer conditions. Briefly, 25 g of grains were milled and extracted with 100 ml acetonitrile:water (84:16 v/v) and shaken for 30 min. The suspension was then filtered through Whatman N • 1 and 5 ml were transferred to the column and cleaned by push-through. After cleaning the filtered, 2 ml were taken and evaporated to dryness by N 2 . The sample was then redissolved in 400 l of mobile phase (methanol:water, 12:88) for HPLC quantification. Quantification was relative to external standards of DON (Biopure, Romer; 0.5, 1, 2 and 4 g/ml). DON concentration was determined by liquid chromatography using the methodology described by Palazzini et al. (2007) .

Palazzini J.M., Dunlap C.A., Bowman M.J, & Chulze S.N. (2016). Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles. Microbiological research, 192.

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Seed Germination of Limonium mansanetianum

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Seed were collected from the main population of Limonium mansanetianum in Villanueva de Castellón (Valencia, Spain) in autumn of 2009. The estimated size of the main populations is around 37,300 individuals (Navarro et al. 2010) . Seeds were manually separated from inflorescence and healthy seeds selected (36-38% of the inflorescence), placed in paper envelopes, and dry-stored in a refrigerator at 4ºC until their use. Germination tests were performed in spring of 2010. The seeds were sterilized with 0.5% sodium hypochloride (NaClO) solution for two minutes and then washed several times with distilled water. Germination seeds were carried out using 9 cm diameter Petri-dishes on two layers of filter paper Whatman nº 1 moistened to saturation with distilled water or test solution. Four replicates of 25 seeds each were used for each treatment. Seeds were considered to be germinated at the emergence of the radicle.

Fos M., Alfonso L., Ferrer‐Gallego P.P., & Lumbreras E.L. (2020). Effect of salinity, temperature and hypersaline conditions on the seed germination inLimonium mansanetianuman endemic and threatened Mediterranean species. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 155(1), 165-171.

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Substrate pH Pretreatment for Fermentation

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To correct the pH of each substrate, a pretreatment was carried out. For this, 50 g, the pulp, or husk, was resuspended in 1 L of distilled water containing KOH 0.06% (w/v) and sterilization was carried out at 121℃/30 min [18] (link). The extracts were filtered with Whatman N º 1 and used for fermentation medium.

Moreira M.D., Coimbra J.M., Melo M.M., Ribeiro L.S., Reis K.C.d., Schwan R.F., & Silva C.F. (2021). Exploration of Low-Cost Solid Waste Coffee Processing to Bio-Carotenoids Production. ACTA SCIENTIFIC MICROBIOLOGY, 4(9), 125-136.

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