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Aphidicolin

Manufactured by Enzo Life Sciences
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Aphidicolin is a chemical compound used as a tool in laboratory research. It functions as a DNA polymerase inhibitor, preventing the replication of DNA during the cell cycle. This product is intended for use in scientific research applications.

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Lab products found in correlation

Leflunomide, by Merck Group (2 mentions) Progesterone, by Merck Group (2 mentions) A77 1726, by Enzo Life Sciences (2 mentions) Esomeprazole, by Enzo Life Sciences (2 mentions) A77 1726, by Merck Group (2 mentions) Uridine 5 monophosphate, by Merck Group (2 mentions) Cytidine 5 monophosphate, by Merck Group (2 mentions)

2 protocols using aphidicolin

1

Zebrafish Embryo Drug Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type zebrafish embryos were treated with leflunomide from 50% epiboly until 24 hours post-fertilization in E3 embryo media and fixed with 4% Formaldehyde. Chemical libraries screened: LOPAC (n= 1280), ICCBL (n= 480) and NIH clinical collection (n=450). Other chemical used include leflunomide (Sigma L5025), Progesterone (Sigma, P0130), Esomeprazole (Enzo life sciences), Aphidicolin (Enzo life sciences, BML-CC101–0001) and A77 1726 (Enzo Life Sciences, ALX-430–096-M025). For nucleotide rescue experiments, a cocktail of 10 μg/ml uridine 5′-monophosphate (Sigma, UMP) and 10 μg/ml cytidine 5′-monophosphate (Sigma, CMP) was added to A77 1726. For in situ hybridizations, we followed the methods as described by Thisse C, and Thisse, B28 ,29 .
Santoriello C., Sporrij A., Yang S., Flynn R.A., Henriques T., Dorjsuren B., Greig E.C., McCall W., Stanhope M.E., Fazio M., Superdock M., Lichtig A., Adatto I., Abraham B.J., Kalocsay M., Jurynec M., Zhou Y., Adelman K., Calo E, & Zon L.I. (2020). RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nature cell biology, 22(4), 372-379.
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2

Zebrafish Embryo Drug Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type zebrafish embryos were treated with leflunomide from 50% epiboly until 24 hours post-fertilization in E3 embryo media and fixed with 4% Formaldehyde. Chemical libraries screened: LOPAC (n= 1280), ICCBL (n= 480) and NIH clinical collection (n=450). Other chemical used include leflunomide (Sigma L5025), Progesterone (Sigma, P0130), Esomeprazole (Enzo life sciences), Aphidicolin (Enzo life sciences, BML-CC101–0001) and A77 1726 (Enzo Life Sciences, ALX-430–096-M025). For nucleotide rescue experiments, a cocktail of 10 μg/ml uridine 5′-monophosphate (Sigma, UMP) and 10 μg/ml cytidine 5′-monophosphate (Sigma, CMP) was added to A77 1726. For in situ hybridizations, we followed the methods as described by Thisse C, and Thisse, B28 ,29 .
Santoriello C., Sporrij A., Yang S., Flynn R.A., Henriques T., Dorjsuren B., Greig E.C., McCall W., Stanhope M.E., Fazio M., Superdock M., Lichtig A., Adatto I., Abraham B.J., Kalocsay M., Jurynec M., Zhou Y., Adelman K., Calo E, & Zon L.I. (2020). RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nature cell biology, 22(4), 372-379.
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