Mouse primary melanocytes were isolated from the dorsolateral skin of 3.5-day postnatal mice. The skin was washed by PBS and then digested in 0.25% trypsin overnight. The epidermal layer was separated and minced following a centrifugation at 1000 × g for 10 min. Lastly, the cells were cultured in cell culture incubator with 10% CO2 in Medium 254 with melanocyte supplement (Thermo Fisher Scientific Inc., Waltham, MA, USA) for 14 days.
Melanocyte supplement
Manufactured by Thermo Fisher Scientific
Sourced in United States
Melanocyte supplement is a specialized laboratory product designed to support the growth and maintenance of melanocyte cell cultures. It provides essential nutrients and growth factors required for the in vitro cultivation of these pigment-producing cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using melanocyte supplement
1
Isolation of Mouse Primary Melanocytes
2
UVB Exposure and Melanocyte Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All UVB exposure was performed as previously described9 (link). Briefly, cells were washed by phosphate-buffered saline (PBS) and exposed to UVB in the Stratalinker UV chamber (Stratagene, Cedar Creek, TX, USA), in which the emittance of UVB was measured by the UV photometer (UVP LLC, Upland, CA, USA). HPMs were isolated from normal discarded foreskins as previously described9 (link). Isolated HPMs were cultured in Medium 254 with melanocyte supplement (Thermo Fisher Scientific Inc., Waltham, MA, USA). All cell lines used are mycoplasma negative and were authenticated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!