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Soluble 17β estradiol e2

Manufactured by Merck Group
Sourced in United States

Soluble 17β-Estradiol (E2) is a laboratory reagent produced by Merck Group. It is a form of the naturally occurring estrogen hormone, 17β-estradiol, which is water-soluble. The core function of this product is to serve as a research tool for scientific investigations, providing a consistent and reliable source of this important biomolecule.

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2 protocols using soluble 17β estradiol e2

1

Fluorescent Labeling of Ion Channels

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Membrane impermeable E2-BSA-FITC and soluble 17β-Estradiol (E2) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Extracellular rabbit anti-MaxiK-α was purchased from Alomone Labs (Jerusalem, Israel), Intracellular goat anti-KCa1.1 antibody from Santa Cruz Biotechnology (Dallas, TX, USA) and extracellular rabbit anti-MaxiK-β1 from Novus Biologicals (Littleton, CO, USA). Nuclear marker 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) and secondary antibodies anti-rabbit Alexa Fluor 488, 568 and anti-goat Alexa Fluor 488 were acquired from Invitrogen (Waltham, MA, USA).
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2

Membrane Protein Localization Assay

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Methyl-β-cyclodextrin (MβCD), water-soluble cholesterol (CLR), membrane-impermeant E2-BSA-FITC, and soluble 17β-Estradiol (E2) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Extracellular rabbit anti-MaxiK-α was purchased from Alomone Labs (Jerusalem, Israel) and extracellular rabbit anti-MaxiK-β1 from Novus Biologicals (Littleton, CO, United States). Secondary antibodies anti-rabbit Alexa Fluor 488, 568, and nuclear marker 4ʹ,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) were acquired from Invitrogen (Waltham, MA, United States).
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