Levels of IFNγ, TNFα, IL-12p70, IL-6 in culture supernatants were measured using commercially available kits (EMC101g.96, EMC102a.96, EMC006.96, and EMC004.96, respectively, NeoBioscience, Shenzhen, China) according to the manufacturer’s instructions. The absorbance was recorded using a microplate reader (Thermo Scientific) at 450 nm.
Emc101g 96
Manufactured by Neobioscience
Sourced in China
The EMC101g.96 is a laboratory equipment product. It is a multi-channel electrochemical measurement device capable of conducting up to 96 simultaneous electrochemical experiments.
Automatically generated - may contain errors
2 protocols using emc101g 96
1
Quantifying Cytokine Levels in Cell Cultures
2
Measuring Cytokine and IgE Levels
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Cytokine concentrations in BALF were measured with commercial enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions. ELISA kits were used to measure interferon (IFN)-γ (EMC101g.96, NeoBioscience, Shenzhen, China), interleukin (IL)-4 (EMC003.96, NeoBioscience, Shenzhen, China), IL-5 (EMC108.96, NeoBioscience, Shenzhen, China), IL-13 (EMC124.96, NeoBioscience, Shenzhen, China), IL-10 (EMC005.96, Neo-Bioscience, Shenzhen, China) and transforming growth factor (TGF)-β (EMC107b.96, NeoBioscience, Shenzhen, China). OVAspecific IgE was assessed by ELISA (439807, BioLegend, San Diego, CA). HDM-specific IgE expression was assessed using a modified protocol. 19 In brief, HDM was coated onto 96-well plates (2.5 μg/well) and incubated overnight at 4 °C. After blocking with 5% FBS in PBS, undiluted serum was added and incubated overnight at 4 °C. After washing, biotin anti-mouse IgE (BD Bioscience-San Jose, CA) was added and incubated at 37 °C for 1 h.
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