Anti rabbit polyclonal antibody against ki 67

Manufactured by GeneTex

Sourced in United States

Anti-rabbit polyclonal antibody against Ki-67. This antibody is generated in rabbits and recognizes the Ki-67 protein, a widely used proliferation marker.

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Lab products found in correlation

Apoptag in situ apoptosis detection kit, by Merck Group (2 mentions)

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Anti-Ki-67 antibody (8 citations)

2 protocols using anti rabbit polyclonal antibody against ki 67

Assessing Tumor Proliferation and Apoptosis

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The histologic sections from individual paraffin-embedded xenograft tumor tissues were deparaffinized and dehydrated. IHC detection of proliferating cells was conducted using anti-rabbit polyclonal antibody against Ki-67 (GeneTex, Inc., Irvine, CA, USA) at a dilution of 1:100. TUNEL assays were conducted for IHC detection of apoptosis using an ApopTag In situ Apoptosis Detection Kit (EMD Millipore, Billerica, MA, USA), in accordance with the manufacturer's protocol.

Nam A.R., Kim J.W., Cha Y., Ha H., Park J.E., Bang J.H., Jin M.H., Lee K.H., Kim T.Y., Han S.W., Im S.A., Kim T.Y., Oh D.Y, & Bang Y.J. (2016). Therapeutic implication of HER2 in advanced biliary tract cancer. Oncotarget, 7(36), 58007-58021.

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Xenograft Tumor Immunohistochemistry and Apoptosis

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Paraffin-embedded xenograft tumor tissues were deparaffinized with xylene and rehydrate with graded ethanol. Immunohistochemistry studies for Ki-67 were conducted by using the anti-rabbit polyclonal antibody against Ki-67 (dilution of 1:100, GeneTex, Irvine, CA) and a terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling assay performed to measure the apoptosis of xenografts using ApopTag In situ Apoptosis Detection Kit (Chemicon International, Temecula, CA) following the manufacturer’s protocol.

Lee M., Lee K.H., Min A., Kim J., Kim S., Jang H., Lim J.M., Kim S.H., Ha D.H., Jeong W.J., Suh K.J., Yang Y.W., Kim T.Y., Oh D.Y., Bang Y.J, & Im S.A. (2018). Pan-Pim Kinase Inhibitor AZD1208 Suppresses Tumor Growth and Synergistically Interacts with Akt Inhibition in Gastric Cancer Cells. Cancer Research and Treatment : Official Journal of Korean Cancer Association, 51(2), 451-463.

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