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2 protocols using sodium dihydrogen phosphate dihydrate

1

Synthesis and Analysis of Sulfur Compounds

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UPLC/MS grade water, methanol and formic acid (>99.98%) were purchased from Biosolve Chimie, Dieuze, France; deuterium (99.8% D), dithiotreitol (98%), (E)-2-hexenal (98%), 2-hexyn-1-ol (≥97%), Boc-Cys-OH (≥98.5%), (Boc-Cys-OH)2 (≥98%), ethanol-OD (≥99%), hydrochloric acid (37%), L-glutathione reduced (98%), Lindlar catalyst, manganese(IV) oxide (≥99%), mesityl oxide (≥90%), mesityl oxide d10 (>98% D), triethylamine (≥99%) were purchased from Sigma-Aldrich, St Quentin Fallavier, France, and H-Cys-Gly-OH (98%), H-Glu(Cys-OH)-OH (98%)from Bachem, Bubendorf, Suisse; triethylsilane (≥98%) was obtained from TCI Europe, Zwijndrecht, Belgium; while di-sodium hydrogen phosphate (≥99%), sodium carbonate (≥99%), sodium dihydrogen phosphate dihydrate (≥99%)and trifluoroacetic acid (≥99.9%) were purchased from Carl Roth, Karlsruhe, Germany.
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2

Analytical Techniques for Brewing Ingredients

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Isohop ® (containing 30.31% iso-α-acids) was obtained from BarthHaas UK Ltd. (Tonbridge, United Kingdom). Disodium hydrogen phosphate dihydrate (≥ 99.5%), di-sodium hydrogen phosphate dodecahydrate (≥ 99%), DL-methionine (≥ 98%), ethanol (96%), methanol ROTISOLV ® HPLC gradient, L-histidine CELLPURE ® (≥ 99%), L-glutamine CELLPURE ® (≥ 99%), ortho-phosphoric acid (≥ 85%), potassium dihydrogen phosphate (≥ 98%), potassium hydroxide (≥ 85%), sodium dihydrogen phosphate dihydrate (≥ 99%) and trifluoroacetic acid were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Acetonitrile gradient grade for liquid chromatography LiChrosolv ® , albumin fraction V (from bovine serum) (≥ 97%), D(-)-fructose, glycine (for analysis), ninhydrin (ACS, Reag. Ph Eur), potassium iodate (ACS, ISO, Reag. Ph Eur), and trichloroacetic acid (for analysis) were obtained from Merck KGaA (Darmstadt, Germany). L( +)-arginine (≥ 98%), L( +)-leucine (≥ 99%), L( +)-lysine (≥ 98%), L( +)-proline (99%), and L( +)-valine (≥ 99%) were supplied from Thermo Fisher GmbH (Kandel, Germany). All solutions were made with deionized water (Milli-Q ® ) and prepared daily.
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