Rabbit anti gfp polyclonal antibody chip grade ab290

Manufactured by Abcam

Rabbit anti-GFP polyclonal antibody-Chip Grade (ab290) is a primary antibody produced in rabbits and purified. It is designed for use in chromatin immunoprecipitation (ChIP) experiments to detect green fluorescent protein (GFP).

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Lab products found in correlation

Rabbit anti ha monoclonal antibody c29f4, by Cell Signaling Technology (2 mentions) Rabbit anti gst monoclonal antibody 91g1, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Anti-GFP (ab290, (24 citations) Rabbit anti-GFP polyclonal antibody (18 citations) Anti-GFP antibody (ab290) (12 citations) GFP antibody (Ab290, (7 citations) ChIP grade anti-GFP antibody (4 citations) ChIP] grade ab290 (4 citations) Rabbit anti-GFP (ab290), (4 citations) Rabbit polyclonal anti-GFP (ab290) (3 citations) Rabbit anti-GFP antibody ab290 (2 citations) Rabbit polyclonal GFP antibody (2 citations)

2 protocols using rabbit anti gfp polyclonal antibody chip grade ab290

Characterization of LET-502 Antibody Specificity

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The rabbit polyclonal antibody was raised against C. elegans LET-502 N-terminal region corresponding to amino acids 67-417 (You-Long Biotech, Shanghai, China). The resulting purified antibody was tested for specificity on western blots of wild-type, let-502(RNAi), and LET-502-GFP-expressing animals. Other antibodies used in this study were listed below: rabbit anti-HA monoclonal antibody (C29F4) (Cell Signaling Technology, Beverly, MA), rabbit anti-GST monoclonal antibody (91G1) (Cell Signaling Technology, Beverly, MA), mouse anti-b-Actin(C4) monoclonal antibody (sc-47778) (Santa Cruz Biotechnologies, Dallas, TX), rabbit anti-GFP polyclonal antibody-Chip Grade (ab290) (Abcam, Cambridge, UK), goat anti-rabbit IgG (H+L) Alexa Fluor 594 conjugate (8889) (Cell Signaling Technology, Beverly, MA), and goat anti-rabbit IgG (H+L) FITC conjugate (GB22303) (Servicebio, Wuhan, China).

Zhang W., Wang S., Yang C., Hu C., Chen D., Luo Q., He Z., Liao Y., Yao Y., Chen J., He J., Hu J., Xia T., Lin L, & Shi A. (2020). LET-502/ROCK Regulates Endocytic Recycling by Promoting Activation of RAB-5 in a Distinct Subpopulation of Sorting Endosomes. Cell reports, 32(12).

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Immunodetection of Cellular Proteins

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The following antibodies were used in this study: rabbit anti-actin polyclonal antibody (sc-1616-R) (Santa Cruz Biotechnologies, Dallas, TX), rabbit anti-HA monoclonal antibody (C29F4) (Cell Signaling Technology, Beverly, MA), rabbit anti-GST monoclonal antibody (91G1) (Cell Signaling Technology, Beverly, MA) and rabbit anti-GFP polyclonal antibody-Chip Grade (ab290) (Abcam, Cambridge, UK).

Wang P., Liu H., Wang Y., Liu O., Zhang J., Gleason A., Yang Z., Wang H., Shi A, & Grant B.D. (2016). RAB-10 Promotes EHBP-1 Bridging of Filamentous Actin and Tubular Recycling Endosomes. PLoS Genetics, 12(6), e1006093.

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